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Sulfo-NHS-SS-Biotin

现货
Catalog No.
A8005
伯胺反应性的生物素化试剂
组合的产品项目
规格价格库存 数量
10mg
¥ 1,000.00
现货
100mg
¥ 1,500.00
现货
1g
¥ 9,000.00
现货

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Background

Sulfo-NHS-SS-biotin(sulfosuccinimidyl-20(biotinamido)ethyl-1,3-dithiopropionate) is a long-chain cleavable amine-reactive biotinylation reagent. The presence of the negatively charged sulfonate group in the chemical structure of sulfo-NHS-SS-biotin makes it a water-soluble biotinylation reagent that can be directly added to aqueous reactions without prior dissolution of organic solvents. Although no prior dissolution is required, an aqueous stock solution of sulfo-NHS-SS-biotin must be prepared rapidly and used immediately in case of the occurrence of hydrolysis of the active ester. Sulfo-NHS-SS-biotin has been used to react with amine-containing proteins and other molecules forming a complex which further interacts with avidin or streptavidin probes and to purify targeted molecules using affinity chromatography on a column of immobilized avidin or streptavidin.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson  (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL).  Academic Press  (an imprint of Elsevier):  London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.

文献引用

1. Sheridan J.S. Carrington. "Differential Glycosylation of the Inwardly Rectifying Potassium Channel Kir7.1 by G protein-coupled Receptors." Vanderbilt University.2019.
2. Carrington S, Hernandez C, et al. "G protein-coupled receptors differentially regulate glycosylation and activity of the inwardly rectifying potassium channel Kir7.1." J Biol Chem. 2018 Sep 26. pii: jbc.RA118.003238. PMID:30257863
3. Brasher MI, Martynowicz DM, et al. "Interaction of Munc18c and Syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumour cells." J Biol Chem. 2017 Aug 10. pii: jbc.M117.807438. PMID:28798239

Features

• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the N-terminal-amine
• Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Medium length—spacer arm is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain

Chemical Properties

StorageStore at -20°C
该产品在溶液中不稳定,建议现配现用
M.Wt606.7
Cas No.325143-98-4
FormulaC19H27N4NaO9S4
SynonymsBiotin disulfide N-hydroxysulfosuccinimide ester
Solubility≥30.335mg/mL in DMSO, <2.68 mg/mL in EtOH, <2.74 mg/mL in H2O
Chemical Namesodium;1-[3-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethyldisulfanyl]propanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate
SDFDownload SDF
Canonical SMILESC1C(C(=O)N(C1=O)OC(=O)CCSSCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

生物素化方法[1]:

样品

468/EV 细胞和468/uPAR 细胞

溶解方法

可以溶于水中,为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。

反应条件

1 mg/ml, 冰上15分钟

应用

细胞悬液(1.5×106)用预冷的PBS洗三次后与1mg/mL sulfo-NHS-SS-biotin混合,在冰上放置15分钟。用含100 mmol/L谷氨酸的PBS能够终止生物素化反应。细胞经过PBS清洗后,用含蛋白酶抑制剂的RIPAh缓冲液(20 mmol/L sodium phosphate, 150 mmol/L NaCl (pH 7.4), 1% NP40, 0.1% SDS, and 0.5% deoxycholic acid)重悬。生物素化的膜蛋白用链霉亲和素琼脂糖拉下来,用SDS样品缓冲液洗脱出来,经过SDS-PAGE,电转到PVDF膜上, 最后用抗体进行杂交。

References:

[1]. Minji Jo, Boryana M. Eastman, Drue L. Webb, et al. Cell Signaling by Urokinase-type Plasminogen Activator Receptor Induces Stem Cell-like Properties in Breast Cancer cells . Cancer Res , 2010;70:8948-8958

生物活性

描述 Sulfo-NHS-SS-Biotin是一种水溶性的生物素化试剂。
靶点            
IC50            

质量控制

化学结构

Sulfo-NHS-SS-Biotin

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Sulfo-NHS-SS-Biotin

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