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Sulfo-NHS-LC-Biotin

Catalog No.
A8003
伯胺反应性的生物素化试剂
组合的产品项目
规格价格库存 数量
10mg
¥ 1,000.00
现货
100mg
¥ 1,500.00
现货
1g
¥ 8,000.00
现货

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Background

Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent. As a water-soluble analog of NHS-LC-biotin, Sulfo-NHS-LC-biotin contains a negatively charged sulfonate group on the NHS ring structure, which creates sufficient polarity within the molecule and allows it to be directly added into aqueous reactions without prior dissolution of organic solvents. Sulfo-NHS-LC-biotin covalently binds to amine groups in proteins and other molecules through its NHS ester forming an amide linkage and releasing N-Hydroxysuccinimide. Sulfo-NHS-LC-biotin has a greater length (22.4 A) between a covalently modified molecule and the bicyclic biotin rings than NHS-biotin (13.5 A), which provides the probes a greater opportunity to reach the binding sites deeply buried inside the surface plane of proteins.

Reference

Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson  (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL).  Academic Press  (an imprint of Elsevier):  London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.

文献引用

1.Wu Z, He D, et al. "A bimodal (SERS and colorimetric) aptasensor for the detection of Pseudomonas aeruginosa." Mikrochim Acta. 2018 Oct 31;185(11):528. PMID:30382404
2.Shu F, Chen J, et al. "Cholesterol Crystal-Mediated Inflammation Is Driven by Plasma Membrane Destabilization." Front Immunol. 2018 May 29;9:1163. PMID:29896195
3.Lorin, Charlotte, Isabelle Vögeli, and Ernst Niggli. "Dystrophic cardiomyopathy: role of TRPV2 channels in stretch-induced cell damage." Cardiovascular research 106.1 (2015): 153-162. PMID:25616416

Features

• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the N-terminal-amine
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Medium length—spacer arm is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass

Chemical Properties

StorageStore at -20°C
该产品在溶液中不稳定,建议现配现用
M.Wt556.6
Cas No.127062-22-0
FormulaC20H29N4NaO9S2
SynonymsBiotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
Solubility>27.8mg/mL in DMSO
Chemical Name2,5-dioxo-1-[6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanoyloxy]pyrrolidine-3-sulfonic acid;sodium
SDFDownload SDF
Canonical SMILESC1C(C(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)O.[Na]
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

生物素化方法[1]:

样品

心肌细胞

溶解方法

可溶于水,DMSO或DMF。

反应条件

0.5 mg/ml, 37℃ 孵育2小时

应用

Sulfo-NHS-LC-Biotin 在PBS中溶解,浓度为0.5 mg/ml,37℃下,将它与心肌细胞孵育2小时。孵育完成后,细胞先用含100mM甘氨酸的预冷PBS洗两遍,再用冷的PBS洗两遍去除没有结合的生物素。100ul的链霉亲和素琼脂糖树脂与100ug的样品在4℃下孵育60分钟后能够捕获到生物素化的蛋白质。60℃ 下,链霉亲和素捕获的生物素化蛋白用含40 mg/mL二硫苏糖醇的Laemmli 2X 样品缓冲液洗脱30分钟,最后用相应的抗体进行免疫印迹分析。

References:

[1]. Charlotte Lorin, Isabelle Vigeli, Ernst Niggli. Dystrophic cardiomyopathy - role of TRPV2 channels in stretch-induced cell damage. Cardiovascular Research, 2015.

生物活性

描述 Sulfo-NHS-LC-Biotin是一个水溶性的生物素化试剂,将生物素共价结合在伯胺上。
靶点            
IC50            

质量控制

化学结构

Sulfo-NHS-LC-Biotin

相关生物数据

Sulfo-NHS-LC-Biotin

相关生物数据

Sulfo-NHS-LC-Biotin

相关生物数据

Sulfo-NHS-LC-Biotin