Iodoacetyl-LC-Biotin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Iodoacetyl-LC-Biotin全名为N-iodoacetyl-N-biotinylhexylenediamine，是一种不溶于水的巯基反应性生物素化试剂，需要合适的溶剂，包括二甲基亚砜（DMSO）和二甲基甲酰胺（DMF），在加入到含水反应之前需要先将其溶解。Iodoacetyl-LC-Biotin含有一个生物素双环结构，一个1,6-二氨基己烷间隔臂，连接到生物素的戊酸侧链，臂末端的碘乙酰基提供巯基反应，Iodoacetyl-LC-Biotin末端的碘乙酰基能够与蛋白质和其他分子上的巯基反应形成不可逆键。Iodoacetyl-LC-Biotin的长间隔臂能够使修饰的分子更好地结合至亲和素或链霉亲和素探针。
参考文献：
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
• Protein labelingbiotinylate antibodies or other proteins for use in protein methods
• Intracellular labeling—membrane-permeable, can be used to label inside cells
• Thiol-reactive—reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)
• Iodoacetyl-activated—perform reactions in the dark at pH 7.5 to 8.5 in Tris or borate buffer
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Solubility—water insoluble, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Medium length—spacer arm is 27.1 angstroms; contains hexylenediamine extension
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 510.43 |
| Cas No. | 93285-75-7 |
| Formula | C18H31IN4O3S |
| Synonyms | N-Biotinyl-N'-(iodoacetyl)-1,6-hexanediamine,Iaa-biotin |
| Solubility | ≥51 mg/mL in DMSO with gentle warming; insoluble in EtOH; insoluble in H2O |
| Chemical Name | 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[6-[(2-iodoacetyl)amino]hexyl]pentanamide |
| SDF | Download SDF |
| Canonical SMILES | C1C2C(C(S1)CCCCC(=O)NCCCCCCNC(=O)CI)NC(=O)N2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 生物素化方法[1]: | |
|
样品 |
IgG |
|
溶解方法 |
可以溶于有机溶剂DMSO或者DMF。 |
|
反应条件 |
4mM, 室温90min |
|
应用 |
免疫球蛋白G（26.7 nmol）用1ml含0.1M磷酸钠和5Mm EDTA，pH 6的缓冲液溶解。在此溶液中，加入DTT使得其在溶液中的浓度为50mM，37℃室温孵育90min，然后冷却到室温。多余的DTT用pH 8.3，50mM Tris通过分子筛层析法去除，5mM EDTA用作洗脱液。随后对具有还原巯基的IgG进行生物素化，溶于Me2SO的30 μl浓度为4 mM的 iodoacetyl-LC-biotin溶液加入，混合物在室温下避光孵育90min。最后，残留的生物素化试剂和游离的生物素用pH 7.2，0.1mM 的PBS通过72小时透析除去。BCA 蛋白试验用来确定溶液中蛋白的浓度，与IgG共价结合的总biotin 通过链霉素结合试验进行检测。 |
|
References: [1]. Anna Bogusiewicz,Nell I. Mock, and Donald M. Mock. Instability of the biotin–protein bond in human plasma. Analytical Biochemistry 327 (2004) 156–161. |
|
| Description | Iodoacetyl-LC-Biotin是卤代乙酰活化的巯基反应性生物素化试剂。 | |||||
| 靶点 | protein cysteines | molecules containing sulfhydryl groups | ||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
