SYBR Safe DNA Gel Stain
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SYBR Safe DNA凝胶染色剂是用于在琼脂糖或丙烯酰胺凝胶中显示DNA或RNA的高灵敏性染色剂。 SYBR Safe染色剂是溴化乙锭(EB)的危险性较低的替代品,可用于蓝光或紫外线激发。
SYBR Safe DNA凝胶染色剂是满足您所有分子生物学需求的更好的核酸染色试剂。SYBR Safe DNA凝胶染色剂不仅对您和环境更好,对您的样品也更有益。
SYBR Safe染色剂作为浓缩液提供,可以像溴化乙锭溶液一样使用。该染色剂也适用于在凝胶中染色RNA。
相对于用溴化乙锭染色并暴露于UV光的相同片段,使用SYBR Safe染色并用蓝光显现的DNA片段的克隆效率有显著的改善。
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• 减少暴露于高致突变的EB和有害的紫外线。
SYBR Safe DNA凝胶染色剂与溴化乙锭相比具有更低的致突变性,用起来更安全。在使用SYBR Safe染色剂时,您可以通过使用可见蓝光照明,而不是UV照明来进一步降低暴露风险。当执行暴露密集型操作(如凝胶切割)时,这是特别有价值的。
• 通过减少非特异性背景荧光提高灵敏度。
SYBR Safe染色剂通过UV激发或蓝光激发提供核酸可视化的极好灵敏度。 当与核酸结合时,绿色荧光SYBR Safe染色剂的荧光激发最大值在〜280和〜502nm,发射最大值在〜530nm。 此外,当与蓝光照明一起使用时,SYBR Safe染色剂具有比紫外光照射的溴化乙锭染色的凝胶更少的背景荧光。
• 用于所有EB染色应用的替代品,包括RNA染色。
SYBR Safe染色剂作为溶解于DMSO的浓缩液提供,可以像溴化乙锭溶液一样使用,您可以以1:10000的比例稀释进1X TBE or 1X TAE,加入琼脂糖粉末后加热溶解,或者在溶解后尚未凝固的琼脂糖凝胶中按1:10000比例稀释,您也可以选择电泳结束后凝胶可在SYBR Safe染色溶液(使用1X TBE or 1X TAE以1:3300的比例稀)中进行孵育。SYBR Safe染色剂可以在室温下保存在原包装中,以避免过度的曝光。
SYBR Safe染色溶液在较低分子量(100-200 bp)的效能较低,不推荐使用。
Physical Appearance | DMSO溶液 |
Storage | 使用时室温储存,可置于4°C保存半年 |
M.Wt | 504.66 |
Formula | C28H28N2O3S2 |
Solubility | insoluble in EtOH; insoluble in H2O; ≥14.67 mg/mL in DMSO |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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