Biotin Hydrazide
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mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
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Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
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Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Biotin hydrazide是一种生物素衍生物,可用作探针,用于检测蛋白质的羰基化,其是多种疾病的成分。蛋白质羰基化是一种不可逆的翻译后修饰(PTM),由活性氧(ROS)攻击、大量的脂质氧化产物(如α,β不饱和γ-hydroxyalkenals)或非酶糖化所引起,从而导致蛋白功能的损失。Biotin hydrazide是一种优选的羰基反应性探针,不需要额外的催化剂或还原剂。Biotin hydrazide在pH 5.5条件下可以完全并容易地衍生羰基,使其可用于检测生物样品中羰基化的蛋白质。
参考文献:
Kenneth Hensley, Kelly S. Williamson. Protein Carbonyl Determination Using Biotin Hydrazide. Methods in Biological Oxidative Stress. Methods in Pharmacology and Toxicology 2003, pp 195-199
Madian AG, Regnier FE. Proteomic identification of carbonylated proteins and their oxidation sites. J Proteome Res. 2010;9(8):3766-3780.
• Glycoprotein labeling—biotinylate glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins
• Cell surface labeling—biotinylate and isolate cell surface glycoproteins
• Aldehyde-reactive—reacts with aldehydes formed by periodate-oxidation of sugar groups
• Hydrazide-activated—perform reactions at pH 4 to 6 in buffers such as sodium acetate
• Irreversible—forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved
• Solubility—water insoluble, usually dissolved in DMSO before further dilution in aqueous buffers
• Spacer arm length—15.7Å
Storage | Store at -20°C |
M.Wt | 258.3 |
Cas No. | 66640-86-6 |
Formula | C10H18N4O2S |
Synonyms | (+)-Biotin Hydrazide; Biotin-Hz; Hydrazide Biotin; Biotine Hydrazide |
Solubility | insoluble in EtOH; ≥12.9 mg/mL in DMSO with gentle warming; ≥2.98 mg/mL in H2O with ultrasonic |
Chemical Name | 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanehydrazide |
SDF | Download SDF |
Canonical SMILES | C1C2C(C(S1)CCCCC(=O)NN)NC(=O)N2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
生物素化方法[1]: | |
样品 |
人脐带透明质酸 |
溶解方法 |
可溶于DMSO。 糖蛋白以1-5mg / mL的浓度溶解在冷的100mM乙酸钠中。 |
反应条件 |
1 mM, 4℃反应过夜 |
应用 |
在与生物素反应前, 100mg的人脐带透明质酸在0.1 M的 Mes溶液中(pH 5.0)至少溶解24h(4℃) 才能达到最终浓度为1 mg/ml。Biotin hydrazide 用DMSO溶解成100 mM 的储存液,然后将透明质酸溶液加入其中,使得Biotin hydrazide 溶液的终浓度为1 mM。溶于水的浓度为100 mM 的EDAC 加入到透明质酸-biotin 溶液中,使得EDAC 的终浓度为30 mM。 混合溶液4℃过夜搅拌。没有连上的生物素以及EDAC 可以用4M的盐酸胍溶液去除。透析过的生物素化的透明质酸在-20℃下能够保存几个月。 |
References: [1]. Gregory I. Frost and Robert Stern. A Microtiter-Based Assay for Hyaluronidase Activity Not Requiring Specialized Reagents. ANALYTICAL BIOCHEMISTRY 251, 263–269 (1997). |
Description | Biotin Hydrazide是一种生物素化试剂,用于生物素化糖蛋白和糖脂。 | |||||
靶点 | glycoproteins | glycolipids | ||||
IC50 |
质量控制和MSDS
- 批次:
化学结构
![Biotin Hydrazide](http://www.apexbt.com//media/diy/images/struct/A8007.png)
相关生物数据
![Biotin Hydrazide Biotin Hydrazide](http://www.apexbt.com/media/diy/images/wb/A8007_1.jpg)