Biotin Hydrazide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Biotin hydrazide是一种生物素衍生物，可用作探针，用于检测蛋白质的羰基化，其是多种疾病的成分。蛋白质羰基化是一种不可逆的翻译后修饰（PTM），由活性氧（ROS）攻击、大量的脂质氧化产物（如α,β不饱和γ-hydroxyalkenals）或非酶糖化所引起，从而导致蛋白功能的损失。Biotin hydrazide是一种优选的羰基反应性探针，不需要额外的催化剂或还原剂。Biotin hydrazide在pH 5.5条件下可以完全并容易地衍生羰基，使其可用于检测生物样品中羰基化的蛋白质。
参考文献：
Kenneth Hensley, Kelly S. Williamson. Protein Carbonyl Determination Using Biotin Hydrazide. Methods in Biological Oxidative Stress. Methods in Pharmacology and Toxicology 2003, pp 195-199
Madian AG, Regnier FE. Proteomic identification of carbonylated proteins and their oxidation sites. J Proteome Res. 2010;9(8):3766-3780.
• Glycoprotein labeling—biotinylate glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins
• Cell surface labeling—biotinylate and isolate cell surface glycoproteins
• Aldehyde-reactive—reacts with aldehydes formed by periodate-oxidation of sugar groups
• Hydrazide-activated—perform reactions at pH 4 to 6 in buffers such as sodium acetate
• Irreversible—forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved
• Solubility—water insoluble, usually dissolved in DMSO before further dilution in aqueous buffers
• Spacer arm length—15.7Å
| Storage | Store at -20°C |
| M.Wt | 258.3 |
| Cas No. | 66640-86-6 |
| Formula | C10H18N4O2S |
| Synonyms | (+)-Biotin Hydrazide; Biotin-Hz; Hydrazide Biotin; Biotine Hydrazide |
| Solubility | insoluble in EtOH; ≥12.9 mg/mL in DMSO with gentle warming; ≥2.98 mg/mL in H2O with ultrasonic |
| Chemical Name | 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanehydrazide |
| SDF | Download SDF |
| Canonical SMILES | C1C2C(C(S1)CCCCC(=O)NN)NC(=O)N2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 生物素化方法[1]: | |
|
样品 |
人脐带透明质酸 |
|
溶解方法 |
可溶于DMSO。 糖蛋白以1-5mg / mL的浓度溶解在冷的100mM乙酸钠中。 |
|
反应条件 |
1 mM, 4℃反应过夜 |
|
应用 |
在与生物素反应前， 100mg的人脐带透明质酸在0.1 M的 Mes溶液中（pH 5.0）至少溶解24h（4℃） 才能达到最终浓度为1 mg/ml。Biotin hydrazide 用DMSO溶解成100 mM 的储存液，然后将透明质酸溶液加入其中，使得Biotin hydrazide 溶液的终浓度为1 mM。溶于水的浓度为100 mM 的EDAC 加入到透明质酸-biotin 溶液中，使得EDAC 的终浓度为30 mM。 混合溶液4℃过夜搅拌。没有连上的生物素以及EDAC 可以用4M的盐酸胍溶液去除。透析过的生物素化的透明质酸在-20℃下能够保存几个月。 |
|
References: [1]. Gregory I. Frost and Robert Stern. A Microtiter-Based Assay for Hyaluronidase Activity Not Requiring Specialized Reagents. ANALYTICAL BIOCHEMISTRY 251, 263–269 (1997). |
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| Description | Biotin Hydrazide是一种生物素化试剂，用于生物素化糖蛋白和糖脂。 | |||||
| 靶点 | glycoproteins | glycolipids | ||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
