Streptavidin-Cy5
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Physical Appearance | Solution. Streptavidin-Cy5 is supplied in phosphate buffered saline (PBS), pH 7.4, containing 1% BSA, 0.1% sodium azide. |
Storage | Store at 2-8°C. Light exposure should be avoided. Do not freeze. |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Recommended Dilution:
1:20 to 1:50 dilution with PBS containing 10% normal goat serum. Working dilution for specific application should be determined by individual investigator to obtain the best conditions.
1)Q:Streptavidin-Cy3、Streptavidin-Cy5、Streptavidin-FITC三种产品应该如何选择?
A:三种产品的不同点在于荧光颜色不同,您可根据实验需要来进行选择,应尽量减少荧光染料间的光谱重叠。FITC是广泛使用的荧光基团,但其最大的缺点是淬灭快,使用抗淬灭剂可以减轻;Cy3和Cy5比大多数荧光基团更亮,更稳定,背景更弱,二者常在共聚焦显微实验中作多标记;Cy5的缺点是不能用表面荧光显微镜观察,需用具有远红外检测器的共聚焦显微镜观察。
2)Q:免疫荧光实验时背景过高?
A:可能原因及解决方案如下:
(1) 一抗浓度可能过高:将抗体进一步稀释至最佳浓度,并在4°C下进行孵育。
(2) 封闭不完全:延长封闭时间,并考虑更换封闭液。
(3) 洗涤不充分:增加各步骤洗涤时间与次数。
(4) 固定过程引起了自发荧光:如福尔马林/PFA通常会在绿色光谱中产生自发荧光,可尝试使用在红色光谱范围内发光的荧光团。
(5) 荧光显微镜参数设置不当:可通过调节荧光显微镜的参数来降低背景。
3)Q:荧光淬灭快?
A:荧光染色后应在1h内完成观察,放置4°C可保存4h,时间过长,可能会使荧光提前衰退。同时建议选择防荧光淬灭剂进行封片。