Sulfo-NHS-LC-Biotin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sulfo-NHS-LC-biotin [磺基琥珀酰亚胺-6-(生物素)己酸]是一种胺反应性的生物素化试剂。作为NHS-LC-biotin的水溶性类似物,Sulfo-NHS-LC-biotin包含一个存在于NHS环上的带负电荷的磺酸基,使其分子内产生足够的极性,允许其可以直接加入到反应性水溶液中,而不需要提前溶解在有机溶剂中。Sulfo-NHS-LC-biotin通过其NHS酯与蛋白或其它分子中的胺基共价结合形成酰胺键,并释放N-Hydroxysuccinimide。与NHS-biotin (13.5 A)相比,Sulfo-NHS-LC-biotin的共价修饰分子与双生物素环之间的长度更长(22.4 A),使探针更容易到达深埋在蛋白质内部的结合位点。
参考文献:
Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- 1. Haibo Jin, Chen Yang, et al. "Evaluation of Neurotoxicity in BALB/c Mice following Chronic Exposure to Polystyrene Microplastics." Environ Health Perspect. 2022 Oct;130(10):107002. PMID: 36251724
- 2. Xihua Yue, Neeraj Tiwari, et al. "Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner." Commun Biol. 2021 Dec 7;4(1):1370. PMID: 34876695
- 3. Julian Nüchel, Marina Tauber, et al. "An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress." Mol Cell. 2021 Aug 19;81(16):3275-3293.e12. PMID: 34245671
- 4. Jia J, Yue X, et al. "KDEL receptor is a cell surface receptor that cycles between the plasma membrane and the Golgi via clathrin-mediated transport carriers." Cell Mol Life Sci. 2020;10.1007/s00018-020-03570-3. PMID: 32562023
- 5. Wu Z, He D, et al. "A bimodal (SERS and colorimetric) aptasensor for the detection of Pseudomonas aeruginosa." Mikrochim Acta. 2018 Oct 31;185(11):528. PMID: 30382404
- 6. Shu F, Chen J, et al. "Cholesterol Crystal-Mediated Inflammation Is Driven by Plasma Membrane Destabilization." Front Immunol. 2018 May 29;9:1163. PMID: 29896195
- 7. Lorin, Charlotte, Isabelle Vögeli, and Ernst Niggli. "Dystrophic cardiomyopathy: role of TRPV2 channels in stretch-induced cell damage." Cardiovascular research 106.1 (2015): 153-162. PMID: 25616416
• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—do not penetrate the plasma membrane, biotinylates only surface proteins of whole cells
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the N-terminal-amine
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Medium length—spacer arm is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
Storage | Store at -20°C该产品在溶液中不稳定,建议现配现用 |
M.Wt | 556.6 |
Cas No. | 127062-22-0 |
Formula | C20H29N4NaO9S2 |
Synonyms | Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt |
Chemical Name | 2,5-dioxo-1-[6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanoyloxy]pyrrolidine-3-sulfonic acid;sodium |
SDF | Download SDF |
Canonical SMILES | C1C(C(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)O.[Na] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
生物素化方法[1]: | |
样品 |
心肌细胞 |
溶解方法 |
可溶于水,DMSO或DMF。 |
反应条件 |
0.5 mg/ml, 37℃ 孵育2小时 |
应用 |
Sulfo-NHS-LC-Biotin 在PBS中溶解,浓度为0.5 mg/ml,37℃下,将它与心肌细胞孵育2小时。孵育完成后,细胞先用含100mM甘氨酸的预冷PBS洗两遍,再用冷的PBS洗两遍去除没有结合的生物素。100ul的链霉亲和素琼脂糖树脂与100ug的样品在4℃下孵育60分钟后能够捕获到生物素化的蛋白质。60℃ 下,链霉亲和素捕获的生物素化蛋白用含40 mg/mL二硫苏糖醇的Laemmli 2X 样品缓冲液洗脱30分钟,最后用相应的抗体进行免疫印迹分析。 |
References: [1]. Charlotte Lorin, Isabelle Vigeli, Ernst Niggli. Dystrophic cardiomyopathy - role of TRPV2 channels in stretch-induced cell damage. Cardiovascular Research, 2015. |
描述 | Sulfo-NHS-LC-Biotin是一个水溶性的生物素化试剂,将生物素共价结合在伯胺上。 | |||||
靶点 | ||||||
IC50 |
质量控制和MSDS
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