HhAntag
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 约2 μM 到大于30 μM。
HhAntag是一种Hedgehog信号通路拮抗剂。在许多人体组织中，Hedgehog (Hh)信号通路的配体依赖性激活与肿瘤发生相关联。
体外：已在一大组癌细胞系中评估HhAntag对Hh通路的作用。在抑制Hh通路的活性方面，已证实HhAntag比cyclopamine强效约10倍。观察到对HhAntag一定范围的细胞敏感性，作用于生长抑制的IC50值范围是2 μM到大于30 μM。与以前的报告相反，体外未观察到对HhAntag敏感性的组织特异性[1]。
体内：含有原代人异种移植物的小鼠口服给药HhAntag导致显著的生长延迟，在胰腺癌和结肠腺癌模型中平均肿瘤生长抑制分别为29％和48％。此外，抑制肿瘤生长所需的HhAntag剂量与完全抑制肿瘤基质或替代正常组织中内源性Hh靶基因所需的剂量相似，表明这种生长抑制是Hh抑制的特异性结果[1]。
临床试验：N/A
参考文献：
[1] Yauch RL,Gould SE,Scales SJ,Tang T,Tian H,Ahn CP,μMarshall D,Fu L,Januario T,Kallop D,Nannini-Pepe M,Kotkow K,μMarsters JC,Rubin LL,de Sauvage FJ. A paracrine requirement for hedgehog signalling in cancer. Nature.2008 Sep 18;455(7211):406-10.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 450.92 |
| Cas No. | 496794-70-8 |
| Formula | C24H23ClN4O3 |
| Solubility | insoluble in H2O; ≥13.3 mg/mL in DMSO; ≥52.3 mg/mL in EtOH with gentle warming |
| Chemical Name | N-[4-chloro-3-[6-(dimethylamino)-1H-benzimidazol-2-yl]phenyl]-3,5-dimethoxybenzamide |
| SDF | Download SDF |
| Canonical SMILES | CN(C)C1=CC2=C(C=C1)N=C(N2)C3=C(C=CC(=C3)NC(=O)C4=CC(=CC(=C4)OC)OC)Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
