AT13387
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AT13387是通过基于高通量X射线晶体片段平台发现的一种非格尔德霉素的热休克蛋白90（HSP90）抑制剂。HSP90是一种分子伴侣，可调控细胞生长和存活的信号转导途径。AT13387可导致客户蛋白降解、信号抑制，并可引发细胞周期停滞和细胞凋亡。由于结构上与格尔德霉素无关，该物质具有结合HSP90的高亲和性（Kd = 0.5 nM），并具有体外细胞毒性，EC50中位值为41 nM。在异种移植的研究中，AT13387具有肿瘤特异的长效保持能力，显示其可能不需频繁给药。然而，初步结果显示AT13387对实体瘤和白血病模型的抗肿瘤活性与alvespimycin相似。
参考文献：
Min H. Kang, C. Patrick Reynolds, Peter J. Houghton, Denise Alexander, Christopher L. Morton, E. Anders Kolb, Richard Gorlick, Stephen T. Keir, Hernan Carol, Richard Lock, John M. Maris, Amy Wozniak, and Malcolm A. Smith. Initial Testing (Stage 1) of AT13387, an HSP90 Inhibitor, by the Pediatric Preclinical Testing Program. Pediatr Blood Cancer. 2012; 59(1): 185-188
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 409.5 |
| Cas No. | 912999-49-6 |
| Formula | C24H31N3O3 |
| Solubility | insoluble in H2O; ≥13.25 mg/mL in DMSO; ≥47.7 mg/mL in EtOH with ultrasonic |
| Chemical Name | (2,4-dihydroxy-5-propan-2-ylphenyl)-[5-[(4-methylpiperazin-1-yl)methyl]-1,3-dihydroisoindol-2-yl]methanone |
| SDF | Download SDF |
| Canonical SMILES | CC(C)C1=C(C=C(C(=C1)C(=O)N2CC3=C(C2)C=C(C=C3)CN4CCN(CC4)C)O)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | AT13387是一种合成的口服生物可利用的小分子热休克蛋白90（Hsp90）抑制剂，在A375细胞中IC50值为18 nM。 | |||||
| 靶点 | Hsp90 | |||||
| IC50 | 18 nM (in A375 cells) | |||||
质量控制和MSDS
- 批次:
化学结构
