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Indirubin

现货
Catalog No.
B3672
细胞周期蛋白依赖性激酶和GSK-3β抑制剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 500.00
现货
5mg
¥ 400.00
现货
25mg
¥ 1,800.00
现货
100mg
¥ 4,700.00
Ship with 10-15 days
250mg
¥ 6,800.00
Ship with 10-15 days

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Background

Interferon-γ is thought to be a member of the inflammatory cytokine family. Because of its augmentation of inflammatory reactions through biological activities. Indirubin, the active ingredient of Danggui Longhui Wan, has inhibitory effects on the production of interferon-γ.

In vitro: Indirubin exerted its inhibitory effects not only on interferon-γ production by human myelomonocytic HBL-38 cells but also on interferon-γ and interleukin-σ production by murine splenocytes with no influence on the proliferation of either cells [1].

In vivo: Because of Indirubin’s inhibitory activity on interferon-γ production, the authors further investigated the effects of indirubin on 2,4,6-trinitro-l-chlorobenzene-induced delayed-type hypersensitivity. When injected intraperitoneally, indirubin significantly inhibited the ear swelling of TNCB-elicited mice. Moreover,the amount of interferon-γ in the culture supernatants of elicited mouse lymphocytes was inhibited by indirubin treatment [1].

Clinical trials: Several clnical trials have been conducted to study the efficacy of indirubin for the treatments of psoriasis and Acute Promyeloid Leukemia .

Reference:
[1] Kunikata T, Tatefuji T, Aga H, Iwaki K, Ikeda M, Kurimoto M.   Indirubin inhibits inflammatory reactions in delayed-type hypersensitivity. Eur J Pharmacol. 2000 Dec 20;410(1):93-100.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt262.26
Cas No.479-41-4
FormulaC16H10N2O2
SolubilitySoluble in DMSO > 10 mM
SDFDownload SDF
Canonical SMILESO=C1/C(NC2=CC=CC=C21)=C3C4=CC=CC=C4NC\3=O
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验 [1]:

细胞系

HBL-38和HPB-ALL细胞

制备方法

在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液,一般步骤如下:请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。

反应条件

0 ~ 1000 ng/mL;持续24小时

实验结果

在HBL-38细胞中,Indirubin (10 ng/mL) 显著抑制干扰素γ合成,但对细胞增殖无影响。Indirubin的IC50值为0.15 ng/mL。然而,在HPB-ALL细胞中,Indirubin不影响植物凝集素诱导的白细胞介素4合成以及细胞增殖。

动物实验 [1]:

动物模型

TNCB诱导的迟发型超敏反应小鼠模型

给药剂量

20 mg/day;腹腔注射;致敏前4天至后4天

实验结果

在单次致敏实验中,Indirubin于第24小时显著抑制TNCB诱导的迟发型超敏反应小鼠模型的耳朵肿胀。 在重复致敏实验中,Indirubin也抑制耳朵肿胀,但上述作用逐渐降低。同时,Indirubin不会导致体重减轻。

注意事项

请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同,但仍处于实验系统误差的允许范围内。

References:

[1]. Kunikata T, Tatefuji T, Aga H, Iwaki K, Ikeda M, Kurimoto M. Indirubin inhibits inflammatory reactions in delayed-type hypersensitivity. Eur J Pharmacol. 2000 Dec 20;410(1):93-100.

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