Fludarabine Phosphate (Fludara)

磷酸氟达拉滨

Catalog No.

A8317

抑制STAT1激活和DNA合成

组合的产品项目
规格	价格	库存
10mM (in 1mL DMSO)	￥ 454.00	现货
10mg	￥ 627.00	现货
50mg	￥ 1,990.00	现货
200mg	￥ 3,818.00	现货

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背景
文献引用
化学属性
试验操作
生物活性
质量控制

背景

Fludarabine phosphate is a STAT-1 activation inhibitor and a DNA synthesis inhibitor. Fludarabine phosphate is the phosphate salt of a fluorinated nucleotide antimetabolite analog of the antiviral agent vidarabine (ara-A) with antineoplastic activity. Fludarabine phosphate is rapidly dephosphorylated to 2-fluoro-ara-A and then phosphorylated intracellularly by deoxycytidine kinase to the active triphosphate, 2-fluoro-ara-ATP. This metabolite may inhibit DNA polymerase alpha, ribonucleotide reductase and DNA primase, thereby interrupting DNA synthesis and inhibiting tumor cell growth.

文献引用

化学属性

Storage	Store at -20°C
M.Wt	365.21
Cas No.	75607-67-9
Formula	C₁₀H₁₃FN₅O₇P
Synonyms	Fludura
Solubility	insoluble in EtOH; ≥17.6 mg/mL in DMSO; ≥6.7 mg/mL in H2O with gentle warming and ultrasonic
Chemical Name	[(2R,3S,4S,5R)-5-(6-amino-2-fluoropurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl dihydrogen phosphate
SDF	Download SDF
Canonical SMILES	C1=NC2=C(N1C3C(C(C(O3)COP(=O)(O)O)O)O)N=C(N=C2N)F
运输条件	蓝冰运输或根据您的需求运输。
一般建议	不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

试验操作

Cell experiment:[1]
Cell lines	Human T lymphoblastoid cells, CCRF-CEM
Reaction Conditions	0.3 ~ 100 μM fludarabine phosphate for 5 h incubation
Applications	Fludarabine phosphate (0.3 ~ 100 μM) was dose-dependently converted to F-ara-ATP in cells. However, the incorporation of F-ara-ATP into DNA was self-limited. At 0.3 ~ 10 μM fludarabine phosphate, the amount of the F-ara-AMP incorporated into DNA increased in a dose-dependent manner, but could not further increased at fludarabine phosphate concentrations greater than 10 μM.
Animal experiment:[2]
Animal models	Mice bearing P388 leukemia
Dosage form	234 mg/kg Injected intraperitoneally
Applications	Fludarabine phosphate administered as a single dose resulted in fewer cells surviving therapy in mice bearing P388 leukemia, accompanied by a greater percentage of increase in life span (110%) and increased median survival time.
Note	The technical data provided above is for reference only.
	References: 1. Huang P, Chubb S, Plunkett W. Termination of DNA synthesis by 9-beta-D-arabinofuranosyl-2-fluoroadenine. A mechanism for cytotoxicity. Journal of Biological Chemistry, 1990, 265(27): 16617-16625. 2. Avramis VI, Plunkett W. Metabolism and therapeutic efficacy of 9-beta-D-arabinofuranosyl-2-fluoroadenine against murine leukemia P388. Cancer Research, 1982, 42(7): 2587-2591.