切换导航

V5 Epitope Tag Peptide

现货
Catalog No.
A6005
多肽序列GKPIPNPLLGLDST
组合的产品项目
规格价格库存 数量
5mg
¥ 1,200.00
现货
25mg
¥ 5,440.00
现货

电话: 021-55669583

邮箱: sales@apexbio.cn

全球经销商

Background

The recognized V5 epitope represents 95GKPIPNPLLGLDST108 of RNA polymerase α subunit of simian parainfluenza virus type 5. This short peptide sequence was chosen because high-affinity antibodies can be reliably produced in many different species1.

A 14-amino-acid V5 epitope derived from simian parainfluenza virus type 5 (Southernet al., 1991) was inserted into the C terminus of the capsid protein (before the stop codon ofORF2) to construct a recombinant marker virus. We demonstrated that the V5 epitope was displayed on the surface ofthe capsid protein. Furthermore, the recombinant marker virus behaved similarly to the parental virus in vitro and in mice, and could be differentiated from the parental virus via polymerase chain reaction (PCR) and serological methods2, 3.

A V5/His-epitope tag was added to the amino terminus of each coding sequence to distinguish the plasmid-encoded protein from endogenous LRRK2. Plasmids were transiently transfected into COS7 cells, and cell lysates were analyzed by SDS-PAGE and Western blotting with antibodies specific for the V5 epitope tag or LRRK24.

References:
1. Zhou,W.H.,Dong, L.L., Ginsburg,D.,Bouhassira, E.E., Tsai,H.M.,2005. Enzymatically active ADAMTS 13 variants are not inhibited by anti-ADAMTS 13 autoantibodies: a novel therapeutic strategy? J. Biol. Chem. 280, 39934–39941.
2.  Southern,J.A.,Young,D.F.,Heaney, F.,Baumgartner,W.K.,Randall,R.E.,1991.Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72,1551–1557
3. L. Huang, Y. Lu et al. Construction and biological characterisation of recombinant porcine circovirus type 2 expressing the V5 epitope tag. Virus Research. 161 (2011) 115–123
4. D. MacLeod,J. Dowman, The Familial Parkinsonism Gene LRRK2Regulates Neurite Process Morphology. Neuron 52, 587–593, 2006

文献引用

1. Yu Z, Surface LE, et al. "Identification of a transporter complex responsible for the cytosolic entry of nitrogen-containing-bisphosphonates." Elife. 2018 May 10;7. pii: e36620. PMID:29745899

Chemical Properties

Physical AppearanceA solid
StorageDesiccate at -20°C
M.Wt1421.64
FormulaC64H108N16O20
SynonymsH-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-OH
Solubility≥71.082mg/mL in DMSO, ≥107.2 mg/mL in EtOH, ≥55.4 mg/mL in H2O
Chemical NameV5 Epitope Tag
SDFDownload SDF
Canonical SMILESCCC(C)C(C(=O)N1CCCC1C(=O)NC(CC(=O)N)C(=O)N2CCCC2C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CC(C)C)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(C(C)O)C(=O)O)NC(=O)C3CCCN3C(=O)C(CCCCN)NC(=O)CN
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

生物活性

描述 V5 Epitope Tag Peptide是猿猴病毒5(simian virus 5)的副粘病毒P和V蛋白中存在的表位衍生而来的标签肽。
靶点 Anti-V5 tag antibody          
IC50            

质量控制

化学结构

V5 Epitope Tag Peptide

相关生物数据

V5 Epitope Tag Peptide