Catalog No. A6002

DYKDDDDK tag Peptide

FLAG 标签肽，也即DYKDDDDK肽

规格	价格	货期	数量
4mg	￥636.00 ￥445.20	现货
25mg	￥2863.00 ￥2004.10	现货

CAS号：98849-88-8纯度：98.19%

仅用于科研，不用于诊疗。未经明确授权不得转售。

021-55669583sales@apexbio.cnAPExBIO

特色产品

Annexin V-FITC/PI Apoptosis Kit

用于双染检测细胞凋亡和坏死

- 在10-20分钟内完成染色
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞

Phos binding reagent (Phosbind) acrylamide

分离和检测磷酸化/非磷酸化蛋白

- 新型的磷酸盐结合标签和功能分子
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白

Cell Counting Kit-8 (CCK-8)

用于细胞增殖和细胞毒性试验

- 比MTT，MTS或WST-1更敏感
- 对细胞无毒性
- 步骤更简单，无需有机溶剂

Cy5 TSA Fluorescence System Kit

Cy5荧光标记的酪胺信号放大系统

- 检测ICC/IHC/ISH中的低丰度靶点
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率

EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)

用于观察mRNA运送，定位，翻译

- 具有Cap1结构的荧光素酶mRNA
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰

HotStart™ 2X Green qPCR Master Mix

用于荧光定量目标DNA或cDNA

- 通过热启动机制优化扩增特异性
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单

HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus

高效的Cy5修饰RNA转录试剂盒

- 适合原位/Northernblot杂交实验
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量

产品描述产品性质产品应用生物相关数据质量控制操作说明产品引用文献摩尔浓度计算器

产品描述

FLAG标签肽（DYKDDDDK）在重组蛋白表达中用作表位标签，以便于检测和纯化。FLAG标签肽用于从抗FLAG M1和M2亲和树脂（K4628）中温和洗脱FLAG融合蛋白。典型工作浓度为100 μg/ml。FLAG标签肽无法洗脱3X FLAG融合蛋白。对于此应用，请使用3X FLAG肽（A6001）。

参考文献：
1. A. Einhauer, A. Jungbauer. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochem. Biophys. Methods 49 2001 455–465.
2. Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A 1981;78:3824–8.
3. Hopp TP. Protein surface analysis: methods for identifying antigenic determinants and other interaction sites. J Immunol Methods 1986;88:1–18.
4. Power BE, Ivancic N, Harley VR, Webster RG, Kortt AA, Irving RA, et al. High-level temperature-induced synthesis of an a

产品性质

产品有效期	12个月
物理外观	固体
CAS号	98849-88-8
分子式	C₄₁H₆₀N₁₀O₂₀
分子量	1012.97
化学名称	FLAG Peptide
溶解度	≥50.65 mg/mL in DMSO; ≥210.6 mg/mL in H2O; ≥34.03 mg/mL in EtOH
SMILES	C1=CC(=CC=C1CC(C(=O)NC(CCCCN)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CCCCN)C(=O)O)NC(=O)C(CC(=O)O)N)O
存储条件	-20°C
运输条件	蓝冰
注意事项	如需配成储存母液，请分装保存，避免反复冻融。

产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)

IC50和靶点

生物活性描述

FLAG标签肽是一个含有肠激酶酶切位点的8肽，可用于重组蛋白的纯化。

生物相关数据

质量控制

请选择批次查看:

纯度 = 99.90%

COA-10.pdf HPLC-10.pdf MS-10.pdf MSDS.pdf

操作说明

Datasheet.pdf

APExBIO 顾客使用本产品发表的 19 篇科研文献

1. Min Jiang, Conghui Li, et al. "Smg5 enhances oligodendrocyte differentiation via nonsense-mediated mRNA decay of Hnrnpl variant transcripts." Journal of Neuroscience 10 September 2025, e0371252025

2. Tong Zhang, Yifei Lyu, et al. "Bacterial immune activation via supramolecular assembly with phage triggers." Nature. 2026 Feb 4. PMID: 41639456

3. Tim Nicholson-Shaw, Megan E Dowdle, et al. "Human CCR4 deadenylase homolog Angel1 is a Non - Stop mRNA Decay factor." RNA. 2025 Jul 16;31(8):1195-1205. PMID: 40441874

4. M. Yusuf Ali, Hailong Lu, et al. "BicD and MAP7 Collaborate to Activate Homodimeric Drosophila Kinesin-1 by Complementary Mechanisms." Traffic. 2025 Apr-Jun;26(4-6):e70008. PMID: 40384341

5. Alireza Ghanbarpour, Bertina Telusma, et al. "An asymmetric nautilus-like HflK/C assembly controls FtsH proteolysis of membrane proteins." EMBO J. 2025 May;44(9):2501-2513. PMID: 40082723

6. Hui-Chi Tang, Kuang-Lei Tsai, et al. "A Protocol to Purify Human Mediator Complex From Freestyle 293-F Cells." Bio Protoc.2025 Feb 20;15(4):e5185. PMID: 40028032

7. Thomas K Sawyer, Efecan Aral, et al. "Human Saposin B Ligand Binding and Presentation to α-Galactosidase A." bioRxiv. 2024 Apr 4:2024.04.04.584535. PMID: 38617236

8. Takushi Miyoshi, Qianli Zhang, et al. "Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures." Cell Rep. 2021 Feb 2;34(5):108708. PMID: 33535030

9. Denghui Wei, Weixiang Zhan, et al. "RAB31 marks and controls an ESCRT-independent exosome pathway." Cell Res. 2021 Feb;31(2):157-177. PMID: 32958903

10. Ji R, Xu X, et al. "Regulation of adiponectin on lipid metabolism in large yellow croaker (Larimichthys crocea)." Biochim Biophys Acta Mol Cell Biol Lipids. 2020;1865(8):158711. PMID: 32289502

11. Marcum RD, Radhakrishnan I. "Inositol phosphates and core subunits of the Sin3L/Rpd3L histone deacetylase (HDAC) complex up-regulate deacetylase activity." J Biol Chem. 2019 Jul 29. pii: jbc.RA119.009780. PMID: 31358618

12. Ter Beek J, Parkash V, et al. "Structural evidence for an essential Fe-S cluster in the catalytic core domain of DNA polymerase." Nucleic Acids Res. 2019 Jun 20;47(11):5712-5722. PMID: 30968138

13. Wang M, Hu J, et al. "High glucose-induced ubiquitination of G6PD leads to the injury of podocytes." FASEB J. 2019 Feb 20:fj201801921R. PMID: 30785802

14. Ilaria Ceppi, Sean M. Howard, et al. "CtIP promotes the motor activity of DNA2 to accelerate long-range DNA end resection." bioRxiv. 2019 October 01

15. Heredia JD, Park J, et al. "Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning." J Immunol. 2018 Apr 20. pii: ji1800343. PMID: 29678950

16. Zi-Wei Chen, John R Bracamontes, et al. "Multiple Functional Neurosteroid Binding Sites on GABAA Receptors." bioRxiv. 2018 June 29

17. Banks CJ, Rodriguez NW, et al. "Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-mediated Inhibition of Mitochondrial Respiration." Mol Cell Biol. 2017 Jul 24. pii: MCB.00354-17. PMID: 28739857

18. Sarah Zernia, Ronny Frank, et al. "Surface‐Binding Peptide Facilitates Electricity‐Driven NADPH‐Free Cytochrome P450 Catalysis." ChemCatChem. 2017 November19

19. Lopez J, Bessou M, et al. "Mito-priming as a method to engineer Bcl-2 addiction." Nat Commun. 2016 Feb 2;7:10538. PMID: 26833356

摩尔浓度计算器

质量

= 浓度

x 体积

x 分子量

*在配置溶液时，请务必参考产品标签上、MSDS / COA（可在Apexbio的产品页面获得）批次特异的分子量使用本工具。