S Tag Peptide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
S Tag是胰核糖核酸酶A(RNase A)衍生的的寡肽。如果RNase A用枯草杆菌蛋白酶(subtilisin)消化,单肽键被裂解,得到两个保持弱结合的产物,该蛋白被称为核糖核酸酶S,仍具有活性,但两个产物单独不具有酶活性。原RNase A的N端,也称为S-肽,由20个氨基酸组成,只有前15个氨基酸是核糖核酸酶活性所必须的。这15个氨基酸的长肽被称为S15或S-tag。
据报道,富含带电和极性残基的多肽可改善它所连接蛋白的溶解性。而且,多肽单独不能折叠成独特的结构。在DNA水平,S-tag可以附着在任何蛋白的N端或C端。基因表达后,这种标签蛋白可通过商业化的抗体来检测[1]。
参考文献:
1. R.T. Raines et al., The S-Tag Fusion System for Protein Purification. Methods Enzymol. 326, 362-367 (2000)
- 1. Takushi Miyoshi, Qianli Zhang, et al. "Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures." Cell Rep. 2021 Feb 2;34(5):108708. PMID: 33535030
- 2. Hawa Gyamfi. "Protein Engineering of Microbial Ferritins." University of Waterloo. 2019.
Physical Appearance | A solid |
Storage | Desiccate at -20°C |
M.Wt | 1748.91 |
Formula | C73H117N23O25S |
Synonyms | H-Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser-OH |
Solubility | ≥174.9 mg/mL in DMSO; insoluble in EtOH; ≥50 mg/mL in H2O |
Chemical Name | S Tag |
SDF | Download SDF |
Canonical SMILES | CC(C(C(=O)NC(C)C(=O)NC(C)C(=O)NC(C)C(=O)NC(CCCCN)C(=O)NC(CC1=CC=CC=C1)C(=O)NC(CCC(=O)O)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCC(=O)N)C(=O)NC(CC2=CN=CN2)C(=O)NC(CCSC)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)O)NC(=O)C(CCC(=O)O)NC(=O)C(CCCCN)N)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
描述 | S Tag Peptide是胰RNase A衍生的寡肽,用于改善蛋白质的溶解性。 | |||||
靶点 | anti-S-Tag antibody | |||||
IC50 |
质量控制和MSDS
- 批次: