Forskolin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC 50:41 nM,作用于I型腺苷酸环化酶。
环磷酸腺苷(cAMP)是一种第二信使,在生物过程中非常重要。Forskolin(毛喉素)是植物毛喉鞘蕊活化腺苷酸环化酶衍生的二萜。腺苷酸环化酶可增加细胞内cAMP的水平。Forskolin的抗炎和抗氧化作用是由于对巨噬细胞激活的抑制,从而减少血栓素B2和超氧化物的水平。该特性使得forskolin成为一种有效的药物,用于治疗心脏病、高血压、糖尿病和哮喘。
体外实验:Forskolin对腺苷酸环化酶的激活导致多种真核细胞内环AMP水平的显著增加。而低浓度forskolin通过增强完整细胞内腺苷酸环化酶的激活,只能少量增加细胞内环AMP。Forskolin也产生依赖于环AMP作为第二信使的细胞内反应[1]。
体内实验:与健康雄性Wistar大鼠相比,评估forskolin慢性给药对streptozotocin诱导的糖尿病雄性Wistar大鼠血糖和氧化应激的影响。在健康和糖尿病大鼠中,在实验结束时,forskolin降低了空腹血糖的水平[2]。
临床试验:一个单盲实验用于比较口服forskolin与吸入倍氯米松(beclomethasone)对轻度或中度持续性成人哮喘的疗效。结果表明,forskolin和倍氯米松治疗组的任何肺功能参数均没有统计学显著的差异。倍氯米松治疗组的患者没有哮喘发作,而forskolin治疗组有一个患者在2个月的研究期间出现了轻度哮喘发作[3]。
参考文献:
[1] Seamon KB, Daly JW. Forskolin: a unique diterpene activator of cyclic AMP-generating systems. J Cyclic Nucleotide Res. 1981;7(4):201-24.
[2] Ríos-Silva M, Trujillo X, Trujillo-Hernández B, Sánchez-Pastor E, Urzúa Z, Mancilla E, Huerta M. Effect of chronic administration of forskolin on glycemia and oxidative stress in rats with and without experimental diabetes. Int J Med Sci. 2014 Mar 11;11(5):448-52.
[3] Huerta M, Urzúa Z, Trujillo X, González-Sánchez R, Trujillo-Hernández B. Forskolin compared with beclomethasone for prevention of asthma attacks: a single-blind clinical trial. J Int Med Res. 2010 Mar-Apr;38(2):661-8.
- 1. Xiaoya An, Guoliang Wang, et al. "Novel cell culture paradigm prolongs mouse corneal epithelial cell proliferative activity in vitro and in vivo." Front Cell Dev Biol. 2021 Jun 30;9:675998. PMID: 34277619
- 2. Emilio J Vélez, Suraj Unniappan. "Nesfatin-1 and nesfatin-1-like peptide suppress growth hormone synthesis via the AC/PKA/CREB pathway in mammalian somatotrophs." Sci Rep. 2020 Oct 7;10(1):16686. PMID: 33028951
- 3. Liu DT, Hong WS, et al. "Upregulation of adamts9 by gonadotropin in preovulatory follicles of zebrafish." Mol Cell Endocrinol. 2019 Oct 2;499:110608. PMID: 31586455
- 4. Zhang H, Wen JJ, et al. "Forskolin reduces fat accumulation in Nile tilapia (Oreochromis niloticus) through stimulating lipolysis and beta-oxidation." Comp Biochem Physiol A Mol Integr Physiol. 2018 Dec 26;230:7-15. PMID: 30593869
- 5. Morel JD, Paatero AO, et al. "Proteomics reveals scope of mycolactone-mediated Sec61 blockade and distinctive stress signature." Mol Cell Proteomics. 2018 Jun 18. pii: mcp.RA118.000824. PMID: 29915147
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 410.5 |
Cas No. | 66575-29-9 |
Formula | C22H34O7 |
Solubility | insoluble in H2O; ≥13.43 mg/mL in EtOH; ≥20.53 mg/mL in DMSO |
Chemical Name | [(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-3-ethenyl-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-5,6,6a,8,9,10-hexahydro-2H-benzo[f]chromen-5-yl] acetate |
SDF | Download SDF |
Canonical SMILES | C[C@]1([C@@H]([C@@H]2O)C(C)(C)CC[C@@H]1O)[C@]3(O)[C@]([C@H]2OC(C)=O)(C)O[C@@](C)(C=C)CC3=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1, 2]: | |
细胞系 |
人源间充质干细胞 |
溶解方法 |
该化合物在DMSO中的溶解度> 10 mM。若配制更高浓度的溶液,一般步骤如下:请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。 |
反应时间 |
0.075-0.2 mM 处理4天或7天; 或10μM |
应用 |
使用不同浓度Forskolin处理人源间充质干细胞4天,抑制其增殖;同时,Forskolin以浓度相关的方式促进人源间充质干细胞表达碱性磷酸酶;除此之外,Forskolin (10 μM)显著地刺激大鼠下丘脑-垂体神经系统释放血管加压素和催产素。 |
动物实验 [1]: | |
动物模型 |
颗粒物植入雄性裸鼠皮下口袋模型 |
剂量 |
0.10 或0.15mM Forskolin |
应用 |
Forskolin(0.10 mM)在体内促进人源间充质干细胞的骨形成。 |
注意事项 |
请于室内测试所有化合物的溶解度。实际溶解度和理论值可能略有不同,这是由实验系统的误差引起的,属于正常现象。 |
References: 1. Doorn, J., Siddappa, R., van Blitterswijk, C. A. and de Boer, J. (2012) Forskolin enhances in vivo bone formation by human mesenchymal stromal cells. Tissue Eng Part A. 18, 558-567 2. Roszczyk, M. and Juszczak, M. (2014) Forskolin-stimulated vasopressin and oxytocin release from the rat hypothalamo-neurohypophysial system in vitro is inhibited by melatonin. Endokrynol Pol. 65, 125-131 |
质量控制和MSDS
- 批次: