MDL 12330A hydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC 50:作用于组胺刺激的腺苷酸环化酶为1.5×10 -5 M [1]。
RMI 12330 A是N,-(顺式-2-苯基环戊基)azacyclotridecan -2-亚胺盐酸盐,据报道其抑制霍乱毒素诱导的肠分泌过多,推测为通过抑制粘膜腺苷酸环化酶。
体外:10 μM至5 mM浓度范围内的RMI 12330 A能有效地抑制大鼠肝脏细胞质膜制备物的腺苷酸环化酶活性。RMI 12330 A在酶的催化位点不应用其竞争性来执行抑制作用。RMI 12330 A对腺苷酸环化酶的抑制活性是由于其与质膜的不可逆结合[2]。
离体:在豚鼠离体的活动心脏中,RMI 12330A抑制所有心功能,IC50为1.1 × 10-6 M,包括收缩力、dP/dt、dF/dt、形成压力、中风和活动性能。正性肌力应答增加心率(staircase),左心房压力上升为负。哇巴因、异丙肾上腺素和组胺不再有正性肌力作用。RMI 12330A抑制后,通过将灌注钙浓度从2.5 mm提高到4.5和6.5 mm,心脏功能可完全恢复[1]。
临床试验:目前尚未进行临床研究。
参考文献:
[1] Grupp G, Grupp IL, Johnson CL, Matlib MA, Rouslin W, Schwartz A, Wallick ET, Wang T, Wisler P. Effects of RMI 12330A, a new inhibitor of adenylate cyclase on myocardial function and subcellular activity. Br J Pharmacol. 1980 Nov;70(3):429-42.
[2] Guellaen G, Mahu JL, Mavier P, Berthelot P, Hanoune J. RMI 12330 A, an inhibitor of adenylate cyclase in rat liver. Biochim Biophys Acta. 1977 Oct 13;484(2):465-75.
- 1. YONGLI ZHAO, NA TANG, et al. "Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin‑angiotensin system." Exp Ther Med. 2020 Aug;20(2):818-829. PMID: 32742325
- 2. Wenwen Zhang, Ruixia Sun, et al. "CaSR participates in the regulation of vascular tension in the mesentery of hypertensive rats via the PLC-IP3/AC-V/cAMP/RAS pathway." Molecular Medicine Reports. August 27, 2019.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 377.01 |
Cas No. | 40297-09-4 |
Formula | C23H36N2·HCl |
Solubility | Soluble in DMSO |
Chemical Name | (E)-N-((1R,2R)-2-phenylcyclopentyl)azacyclotridec-1-en-2-amine hydrochloride |
SDF | Download SDF |
Canonical SMILES | [C@@H](CCC1)(C2=CC=CC=C2)[C@@H]1N/C3=N/CCCCCCCCCCC3.Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |