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(-)-Epigallocatechin gallate (EGCG)

现货
Catalog No.
A2600
抗氧化,抗血管生成和抗肿瘤剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 550.00
现货
100mg
¥ 500.00
现货
500mg
¥ 2,100.00
现货

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Background

(-)-Epigallocatechin gallate (EGCG), the major catechin accounting for 59% of the total catechins in green tea, is a powerful antioxidant as well as an antiangiogenic and antitumor agent. EGCG has been studied for its role in the chemoprevention of a wild range of cancers, including liver, stomach, skin, lung, mammary gland and colon cancers. Study results show that EGCG is able to induce apoptosis, promote cell growth arrest and block carcinogenesis by affecting signal transduction pathways. Moreover, EGCG exhibits inhibition against a variety of viruses, including HCV, HIV-1, HBV, HSV-1, HSV-2, EBV, adenovirus, influenza virus and enterovirus, as well as several enzymes, including DNMTs, proteases and DHFR.

Reference

Singh BN, Shankar S, Srivastava RK. Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochem Pharmacol. 2011; 82(12):1807-1821.

Steinmann J, Buer J, Pietschmann T, Steinmann E. Anti-infective properties of epigallocatechin-3-gallate (EGCG), a component of green tea. Br J Pharmacol. 2013; 168(5):1059-1073

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt458.37
Cas No.989-51-5
FormulaC22H18O11
SynonymsEGCG
Solubility≥22.9mg/mL in DMSO
Chemical Name[(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate
SDFDownload SDF
Canonical SMILESC1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验 [1]:

细胞系

人和大鼠神经祖细胞(NPC)

制备方法

在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液,一般步骤如下:请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。

反应条件

0、1、2、5和10 μM;24或48小时

实验结果

(-)-Epigallocatechin Gallate (EGCG)体外影响人和大鼠NPC发育。EGCG影响迁移距离、迁移模式以及NPC聚集成为神经球的核密度。EGCG结合到细胞外基质(ECM)糖蛋白(层粘连蛋白),防止层粘连蛋白结合整合蛋白β1亚基,从而减少细胞粘附并导致神经胶质细胞排列改变和迁移神经元数目减少。

动物实验 [2]:

动物模型

大鼠膀胱出口部分梗阻(pBOO)(引起损伤的)模型

给药剂量

4.5 mg/kg/day;腹腔注射;2天或30天

实验结果

在第48小时,EGCG缓解pBOO引起的膀胱炎。在第30天,EGCG减少内质网(ER)应激相关的细胞凋亡。此外,在第30天,EGCG改善膀胱顺应性、收缩频率以及炎症。

其他注意事项

请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同,但仍处于实验系统误差的允许范围内。

References:

[1]. Barenys M, Gassmann K, Baksmeier C, Heinz S, Reverte I, Schmuck M, Temme T, Bendt F, Zschauer TC, Rockel TD, Unfried K, W?tjen W, Sundaram SM, Heuer H, Colomina MT, Fritsche E. Epigallocatechin gallate (EGCG) inhibits adhesion and migration of neural progenitor cells in vitro. Arch Toxicol. 2016 Apr 26.

[2]. Hsieh JT, Kuo KL, Liu SH, Shi CS, Chang HC, Lin WC, Chou CT, Hsu CH, Liao SM, Wang ZH, Li CC, Huang KH. Epigallocatechin Gallate Attenuates Partial Bladder Outlet Obstruction-induced Bladder Injury via Suppression of Endoplasmic Reticulum Stress-related Apoptosis-In Vivo Study. Urology. 2016 May;91:242.e1-9.

生物活性

描述 (-)-Epigallocatechin Gallate是绿茶中主要的儿茶素提取物。
靶点 PKC          
IC50            

质量控制

化学结构

(-)-Epigallocatechin gallate(EGCG)