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(-)-Epigallocatechin gallate (EGCG)
抗氧化,抗血管生成和抗肿瘤剂

(-)-Epigallocatechin gallate (EGCG)

产品编号:A2600
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10mM (in 1mL DMSO) ¥550.00 现货
100mg ¥500.00 现货
500mg ¥2,100.00 现货

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Sample solution is provided at 25 µL, 10mM.

质量控制

化学结构

(-)-Epigallocatechin gallate(EGCG)

(-)-Epigallocatechin gallate (EGCG) Dilution Calculator

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(-)-Epigallocatechin gallate (EGCG) Molarity Calculator

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化学性质

CAS号 989-51-5 SDF Download SDF
别名 EGCG
化学名 [(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate
SMILES C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O
分子式 C22H18O11 分子量 458.37
溶解度 ≥22.9mg/mL in DMSO 储存条件 Store at -20°C
物理性状 A solid 运输条件 试用装:蓝冰运输。
其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议 为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

生物活性

描述 (-)-Epigallocatechin Gallate是绿茶中主要的儿茶素提取物。
靶点 PKC          
IC50            

实验操作

细胞实验 [1]:

细胞系

人和大鼠神经祖细胞(NPC)

制备方法

在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液,一般步骤如下:请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。

反应条件

0、1、2、5和10 μM;24或48小时

实验结果

(-)-Epigallocatechin Gallate (EGCG)体外影响人和大鼠NPC发育。EGCG影响迁移距离、迁移模式以及NPC聚集成为神经球的核密度。EGCG结合到细胞外基质(ECM)糖蛋白(层粘连蛋白),防止层粘连蛋白结合整合蛋白β1亚基,从而减少细胞粘附并导致神经胶质细胞排列改变和迁移神经元数目减少。

动物实验 [2]:

动物模型

大鼠膀胱出口部分梗阻(pBOO)(引起损伤的)模型

给药剂量

4.5 mg/kg/day;腹腔注射;2天或30天

实验结果

在第48小时,EGCG缓解pBOO引起的膀胱炎。在第30天,EGCG减少内质网(ER)应激相关的细胞凋亡。此外,在第30天,EGCG改善膀胱顺应性、收缩频率以及炎症。

其他注意事项

请于室内测试所有化合物的溶解度。虽然化合物的实际溶解度可能与其理论值略有不同,但仍处于实验系统误差的允许范围内。

References:

[1]. Barenys M, Gassmann K, Baksmeier C, Heinz S, Reverte I, Schmuck M, Temme T, Bendt F, Zschauer TC, Rockel TD, Unfried K, W?tjen W, Sundaram SM, Heuer H, Colomina MT, Fritsche E. Epigallocatechin gallate (EGCG) inhibits adhesion and migration of neural progenitor cells in vitro. Arch Toxicol. 2016 Apr 26.

[2]. Hsieh JT, Kuo KL, Liu SH, Shi CS, Chang HC, Lin WC, Chou CT, Hsu CH, Liao SM, Wang ZH, Li CC, Huang KH. Epigallocatechin Gallate Attenuates Partial Bladder Outlet Obstruction-induced Bladder Injury via Suppression of Endoplasmic Reticulum Stress-related Apoptosis-In Vivo Study. Urology. 2016 May;91:242.e1-9.

研究更新

1. (-)-Epigallocatechin-gallate (EGCG) stabilize the mitochondrial enzymes and inhibits the apoptosis in cigarette smoke-induced myocardial dysfunction in rats. Mol Biol Rep. 2013 Dec;40(12):6533-45. doi: 10.1007/s11033-013-2673-5. Epub 2013 Nov 7.
Abstract
EGCG prevents cardiac mitochondrial metabolism and apoptosis in CS-exposed rats and exhibits enduring cardio protection at mitochondrial level, where it shelters the activities of TCA cycle enzymes and antioxidant enzymes from CS exposure with concomitant decrease in lipid peroxidation and increase in GSH level, inhibits apoptosis through a series of cellular events, including inhibition of the release of cytochrome c into cytosol, activation of pro-caspase-3, down-regulation of Bax and up-regulation of Bcl-2, and reverses the ultra structural apoptotic alternations of mitochondria and nucleus.
2. The reconstructed skin micronucleus assay in EpiDerm™: reduction of false-positive results - a mechanistic study with epigallocatechin gallate. Mutat Res. 2013 Oct 9;757(2):148-57. doi: 10.1016/j.mrgentox.2013.08.001. Epub 2013 Aug 27.
Abstract
EGCG, which causes positive results in in vitro mammalian genotoxicity assays via oxidative stress and negative results in in vivo MN studies, was used to assess the effect of cell differentiation status on MN induction and significantly induced the expression of genotoxic response related genes in NHEKs after 12h. The Oxidative stress probably caused by EGCG can be eliminated by EpiDermTM under in vitro experimental conditions.
3. Assessment of DNA damage and lipid peroxidation in diabetic mice: effects of propolis and epigallocatechin gallate (EGCG). Mutat Res. 2013 Sep 18;757(1):36-44. doi: 10.1016/j.mrgentox.2013.04.022. Epub 2013 Jul 13.
Abstract
EGCG, a strong antioxidant with possible anti-obesity and anti-diabetic effects, was administered intraperitoneally to alloxan-induced diabetic mice at doses of 50mg/kg body weight for a period of 7 days resulting in a significant increase of body weight and haematological/immunological blood parameters, 100% survival, a significant decrease of lipid peroxidation in liver, kidney and brain tissue, and remarkably reduced DNA damage in peripheral lymphocytes. Due to the demonstrated beneficial effects against diabetes and the associated consequences of free-radical formation in kidney, liver, spleen and brain tissues, EGCG could be used as a dietary supplement potentially contributing to nutritional strategies for the prevention and treatment of diabetes mellitus.
4. Epigallocatechin-gallate (EGCG) regulates autophagy in human retinal pigment epithelial cells: a potential role for reducing UVB light-induced retinal damage. Biochem Biophys Res Commun. 2013 Sep 6;438(4):739-45. doi: 10.1016/j.bbrc.2013.07.097. Epub 2013 Jul 31.
Abstract
EGCG is involved in UVB irradiation-induced autophagy in RPE cells, where the treatment of EGCG significantly reduced the formation of LC3-II and autohagosomes and remarkably relieved UVB irradiation-induced toxicity to RPE cells in an autophagy-dependent manner, and is a potential therapeutic reagent that could be incorporated into the treatment of pathological conditions associated with abnormal autophagy.
5. Green tea epigallocatechin gallate binds to and inhibits respiratory complexes in swelling but not normal rat hepatic mitochondria. Biochem Biophys Res Commun. 2014 Jan 17;443(3):1097-104. doi: 10.1016/j.bbrc.2013.12.110. Epub 2013 Dec 31.
Abstract
EGCG, which is hepatotoxic to humans and animals, barely affected oxidative phosphorylation at 7.5-100 μΜ in normal mitochondria of rats liver but remarkably inhibited RCCs in mitochondria undergoing Ca(2+) overload-induced MPT only if IM integrity was compromised. EGCG promotes Ca(2+) overload-induced MPT after moderate MPT has already commenced and worsens pre-existing mitochondria abnormalities triggering hepatotoxicity since it only targets hepatic RCCs in swelling mitochondrial other than normal mitochondrial.

产品描述

(-)-Epigallocatechin gallate(EGCG)是绿茶中主要的儿茶素成分,占总儿茶素成分的59%,是一种有效的抗氧化剂、抗血管生成和抗肿瘤剂。EGCG已被用于研究其在多种肿瘤的化学预防中的作用,包括肝癌、胃癌、皮肤癌、肺癌、乳腺癌和结肠癌。研究表明,EGCG通过影响信号传导通路,能够诱导细胞凋亡,促进细胞生长停滞和防止癌变。而且,EGCG对许多病毒也具有抑制作用,包括HCV、HIV-1、HBV、HSV-1、HSV-2、EBV、腺病毒、流感病毒和肠病毒,以及几种酶,包括DNMTs、蛋白酶和DHFR。

参考文献:
Singh BN, Shankar S, Srivastava RK.  Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochem Pharmacol. 2011; 82(12):1807-1821.
Steinmann J, Buer J, Pietschmann T, Steinmann E.  Anti-infective properties of epigallocatechin-3-gallate (EGCG), a component of green tea. Br J Pharmacol. 2013; 168(5):1059-1073