Apoptosis Inhibitor
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
细胞凋亡抑制剂是一种新型的细胞凋亡和caspase-3抑制剂,作用于可溶性人Fas配体诱导的人Fas阳性WC8细胞死亡、Etoposide诱导的U937细胞死亡和U937细胞caspase-3激活,IC50值分别为67 μg/ml、130 μg/ml和79 μg/ml[1]。
在哺乳动物细胞凋亡中,caspase-3是一个经常被活化的蛋白酶。它对于某些特定的细胞凋亡过程和细胞死亡是必须的[2]。
Etoposide对caspase-3的体外激活可剂量依赖性地被细胞凋亡抑制剂所抑制。使用重组人caspase-3和DEVD-p-nitroanilide作为底物来证实,即使浓度高达1000 mg/ml,细胞凋亡抑制剂也不直接抑制caspase-3的酶活性[1]。
在给予细胞凋亡抑制剂的鱼中,caspase-3+细胞的数量减小到约为对照组的55%,凋亡神经元(caspase-3+/Hu+)、凋亡新神经元(caspase-3+/BrdU+/Hu+)和凋亡新分裂细胞(caspase-3+/BrdU+)的数量显著下降到对照组值的35-39% [3]。当截肢后的非洲爪蟾幼体立即暴露于20 μM的细胞凋亡抑制剂,被暴露幼体的caspase-3活性水平比对照幼体的水平低20%±1%[4]。
参考文献:
[1]. Tsuda T., Ohmori Y., Muramatsu H., et al. Inhibitory effect of M50054, a novel inhibitor of apoptosis, on anti-Fas-antibody-induced hepatitis and chemotherapy-induced alopecia. European Journal of Pharmacology, 2001, 433:37-45.
[2]. Porter A.G. and Jnicke R.U. Emerging roles of caspase-3 in apoptosis. Cell Death and Differentiation, 1999, 6:99-104.
[3]. Srbulescu R.F. and Zupanc G.K. Inhibition of caspase-3-mediated apoptosis improves spinal cord repair in a regeneration-competent vertebrate system. Neuroscience, 2010, 171(2):599-612.
[4]. Tseng A.S., Adams D.S., Qiu D., et al. Apoptosis is required during early stages of tail regeneration in Xenopus laevis. Dev. Biol., 2007, 301(1):62-9.
Physical Appearance | A solid |
Storage | Store at 4°C |
M.Wt | 236.3 |
Cas No. | 54135-60-3 |
Formula | C13H16O4 |
Solubility | insoluble in H2O; ≥2.57 mg/mL in EtOH with gentle warming and ultrasonic; ≥9.1 mg/mL in DMSO |
Chemical Name | 2-[(2,6-dioxocyclohexyl)methyl]cyclohexane-1,3-dione |
SDF | Download SDF |
Canonical SMILES | C1CC(=O)C(C(=O)C1)CC2C(=O)CCCC2=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
描述 | 细胞凋亡抑制剂是凋亡诱导的细胞渗透性抑制剂。 | |||||
靶点 | Caspase | |||||
IC50 |
质量控制和MSDS
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