AZ 10417808
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AZ 10417808是一种选择性的caspase-3抑制剂，IC50值为14.9 μM [1]。
Caspase-3蛋白是半胱氨酸天冬氨酸蛋白酶（caspase）家族的成员。Caspases的顺序激活在细胞凋亡的执行阶段起重要作用[1]。
AZ10417808（AQZ-1）是一种有效的非肽类小分子caspase-3抑制剂，阻断细胞凋亡的细胞和生化特征[1]。评估AZ 10417808对caspases 1、2、3、6、7、8的抑制活性表明，只有caspases-3被有效地抑制。AZ 10417808完全阻断星形孢菌素诱导的细胞内DEVDase活性，而且这种效应是剂量依赖性的。与对照细胞相比（64 ± 7%），星形孢菌素与10 μM AZ 10417808处理的细胞有92 ± 2%的存活率，表明在SH-SY5Y 细胞培养凋亡模型中，AZ 10417808增加细胞活力[1]。
参考文献：
[1]. Scott CW, Briner CS, Wilkins DE, et al. Novel small molecule inhibitors of caspase-3 block cellular and biochemical features of apoptosis. J Pharmacol Exp Ther, 2003, 304(1): 433-40.
- 1. Chang-Yong Choi, Mai Tram Vo, et al. "Autophagy-competent mitochondrial translation elongation factor TUFM inhibits caspase-8-mediated apoptosis." Cell Death Differ. 2022 Feb;29(2):451-464. PMID: 34511600
- 2. Jiao C, Chen W, et al. "Ganoderma lucidum spore oil induces apoptosis of breast cancer cells in vitro and in vivo by activating caspase-3 and caspase-9." J Ethnopharmacol. 2019 Oct 3:112256. PMID: 31586690
| Physical Appearance | Yellow solid |
| Storage | Store at RT |
| M.Wt | 434.23 |
| Cas No. | 331645-84-2 |
| Formula | C18H13Cl2N5O4 |
| Solubility | <10.86mg/ml in DMSO |
| Chemical Name | 2-(3,4-dichloroanilino)-6-nitro-4-oxo-N-prop-2-enyl-1H-quinazoline-8-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | C=CCNC(=O)C1=C2C(=CC(=C1)[N+](=O)[O-])C(=O)N=C(N2)NC3=CC(=C(C=C3)Cl)Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
相关生物数据
