V5 Epitope Tag Peptide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
被识别的V5抗原表位是simian parainfluenza virus type 5（Southernet al., 1991）的RNA聚合酶α亚基中的序列95GKPIPNPLLGLDST108，由于在许多不同物种中都可以产生高亲和性的抗体，因而选择该短肽序列设计标签肽[1]。
含有14个氨基酸的V5表位被插入衣壳蛋白的C端（在ORF2终止密码子之前），从而构建一个重组标记病毒。V5 epitope被展示在衣壳蛋白的表面。在体外和小鼠中，重组标记病毒与亲本病毒具有相似的活动，可以通过聚合酶链反应（PCR）和血清学的方法加以区分[2，3]。
V5/His-epitope tag加入到每个编码序列的氨基末端用以区分质粒编码的蛋白和内源性LRRK2。质粒瞬时转染COS7细胞后，细胞裂解物用V5 epitope tag或LRRK24特异性的抗体通过SDS-PAGE和Western blotting的方法进行分析。
参考文献：
1. Zhou,W.H.,Dong, L.L., Ginsburg,D.,Bouhassira, E.E., Tsai,H.M.,2005. Enzymatically active ADAMTS 13 variants are not inhibited by anti-ADAMTS 13 autoantibodies: a novel therapeutic strategy? J. Biol. Chem. 280, 39934–39941.
2. Southern,J.A.,Young,D.F.,Heaney, F.,Baumgartner,W.K.,Randall,R.E.,1991.Identi?cation of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics. J. Gen. Virol. 72,1551–1557
3. L. Huang, Y. Lu et al. Construction and biological characterisation of recombinant porcine circovirus type 2 expressing the V5 epitope tag. Virus Research. 161 (2011) 115–123
4. D. MacLeod,J. Dowman, The Familial Parkinsonism Gene LRRK2Regulates Neurite Process Morphology. Neuron 52, 587–593, 2006
- 1. Takushi Miyoshi, Qianli Zhang, et al. "Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures." Cell Rep. 2021 Feb 2;34(5):108708. PMID: 33535030
- 2. Yu Z, Surface LE, et al. "Identification of a transporter complex responsible for the cytosolic entry of nitrogen-containing-bisphosphonates." Elife. 2018 May 10;7. pii: e36620. PMID: 29745899
| Physical Appearance | A solid |
| Storage | Desiccate at -20°C |
| M.Wt | 1421.64 |
| Formula | C64H108N16O20 |
| Synonyms | H-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-OH |
| Solubility | ≥71.08 mg/mL in DMSO; ≥107.2 mg/mL in EtOH; ≥55.4 mg/mL in H2O |
| Chemical Name | V5 Epitope Tag |
| SDF | Download SDF |
| Canonical SMILES | CCC(C)C(C(=O)N1CCCC1C(=O)NC(CC(=O)N)C(=O)N2CCCC2C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CC(C)C)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(C(C)O)C(=O)O)NC(=O)C3CCCN3C(=O)C(CCCCN)NC(=O)CN |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 描述 | V5 Epitope Tag Peptide是猿猴病毒5（simian virus 5)的副粘病毒P和V蛋白中存在的表位衍生而来的标签肽。 | |||||
| 靶点 | Anti-V5 tag antibody | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
