切换导航

KY 02111

现货
Catalog No.
A8213
WNT信号抑制剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 650.00
现货
10mg
¥ 620.00
现货
50mg
¥ 2,480.00
现货
200mg
¥ 6,000.00
现货

电话: 021-55669583

邮箱: sales@apexbio.cn

全球经销商

Background

KY 02111 is a selective inhibitor of Wnt may by targeting the downstream from GSK3βand APC in β-catenin destruction complex [1].

Wnt signaling pathways are a group of signal transduction pathways made of proteins and play an important role in passing signals from outside of a cell through cell surface receptors to the inside of the cell. Wnt signaling initially was identified as a pivotal mediator in carcinogenesis, but has since been recognized for its function in embryonic development, including body axis patterning, cell fate specification, cell proliferation, and cell migration which are necessary for proper formation of important tissues including bone, heart, and muscle [2].

KY 02111 is a potent Wnt inhibitor and has a different selectivity with the reported Wnt inhibitors. When tested with IMR90-1 hiPSCs transfected with TCF receptor plasmids, KY 02111 (1 μM) significantly reduced luciferase activities in a dose-dependent manner by inhibiting canonical WNT signaling pathway. In cardiac colonies on the day 30, KY 02111 showed that nearly 73%-85% of IMR90-1 hiPSCs expressed the cardiac markers [1].

References:
[1].  Minami, I., et al., A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep, 2012. 2(5): p. 1448-60.
[2].  Stamatakou, E., M. Hoyos-Flight, and P.C. Salinas, Wnt Signalling Promotes Actin Dynamics during Axon Remodelling through the Actin-Binding Protein Eps8. PLoS One, 2015. 10(8): p. e0134976.

Chemical Properties

Physical AppearanceA solid
StorageStore at 4°C
M.Wt376.86
Cas No.1118807-13-8
FormulaC18H17ClN2O3S
Solubility≥18.85mg/mL in DMSO, ≥7.06 mg/mL in EtOH with ultrasonic and warming, <2.08 mg/mL in H2O
Chemical NameN-(6-chloro-1,3-benzothiazol-2-yl)-3-(3,4-dimethoxyphenyl)propanamide
SDFDownload SDF
Canonical SMILESCOC1=C(C=C(C=C1)CCC(=O)NC2=NC3=C(S2)C=C(C=C3)Cl)OC
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验 [1]:

细胞系

IMR90-1 hiPSCs

制备方法

在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液,一般步骤如下:请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。

反应条件

10 μM;12或24小时

实验结果

在IMR90-1 hiPSCs中,KY02111 (10 μM) 下调经典Wnt信号途径的靶基因表达 (72.7%)。

References:

[1]. Minami, I., et al., A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep, 2012. 2(5): p. 1448-60.

生物活性

描述 KY02111是hPSCs分化成心肌细胞的促进剂和Wnt信号的抑制剂。
靶点 Wnt Signaling          
IC50            

质量控制

化学结构

KY 02111

相关生物数据

KY 02111
KY 02111 inhibits canonical Wnt pathway

相关生物数据

KY 02111
KY 02111 inhibits canonical Wnt pathway