JSH-23
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
JSH-23是NF-κB转录活性的抑制剂,IC50值为7.1 μM[1]。
在LPS刺激的巨噬细胞RAW 264.7中,JSH-23剂量依赖性地抑制NF-κB的转录活性,而该效应不是由于其细胞毒性。在同样条件下,JSH-23显著减少LPS刺激的NF-κB的DNA结合活性,并减少NF-κB p65的核数量,但并不影响IκB的降解。此外,JSH-23对促炎转录物和酶的表达也具有抑制效果,包括IL-6、IL-1β、COX-2和TNF-α。而且,JSH-23抑制LPS诱导的凋亡染色质浓缩[1]。
参考文献:
[1] Shin HM, Kim MH, Kim BH, Jung SH, Kim YS, Park HJ, Hong JT, Min KR, Kim Y. Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. FEBS Lett. 2004 Jul 30;571(1-3):50-4.
- 1.Linnan Yang, Jing Sun, et al. "Synergetic Functional Nanocomposites Enhance Immunotherapy in Solid Tumors by Remodeling the Immunoenvironment." Advanced Science. 16 February 2019.
- 2.Lee YC, Wang LJ, et al. "ABT-263-induced MCL1 upregulation depends on autophagy-mediated 4EBP1 downregulation in human leukemia cells." Cancer Lett. 2018 Jun 15;432:191-204. PMID:29913235
- 3.Dela Pena-Ponce MG, Jimenez MT, et al. "The Helicobacter pylori type IV secretion system promotes IL-8 synthesis in a model of pediatric airway epithelium via p38 MAP kinase." PLoS One. 2017 Aug 15;12(8):e0183324. PMID:28813514
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 240.34 |
Cas No. | 749886-87-1 |
Formula | C16H20N2 |
Solubility | ≥24 mg/mL in DMSO; insoluble in H2O; ≥17.1 mg/mL in EtOH with ultrasonic |
Chemical Name | 4-methyl-1-N-(3-phenylpropyl)benzene-1,2-diamine |
SDF | Download SDF |
Canonical SMILES | CC1=CC(=C(C=C1)NCCCC2=CC=CC=C2)N |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验[1]: | |
细胞系 |
LPS刺激的RAW264.7细胞 |
溶解方法 |
在DMSO中的溶解度>12mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
0, 1, 3, 10和30 μM |
应用 |
在LPS刺激的RAW264.7细胞中,JSH-23以剂量依赖性方式抑制LPS诱导的SEAP表达,在3μM,10μM和30μM时分别抑制23±3%,68±3%和103±4%。JSH-23也剂量依赖性地降低LPS诱导的NF-κB的DNA结合活性。 JSH-23显示了对LPS诱导的促炎转录物表达的不同抑制效果。 |
动物实验[2]: | |
动物模型 |
顺铂诱导的急性肾损伤(AKI)雄性C57BL / 6小鼠 |
剂量 |
20 mg/kg(顺铂注射前8小时给药10 mg/kg,顺铂注射后第1天和第2天为5mg / kg)或40mg / kg(顺铂注射前8小时给药20mg / kg,顺铂注射后第1天为20mg / kg); 腹膜内(IP)注射 |
应用 |
在顺铂诱导的AKI雄性C57BL / 6小鼠中,JSH-23(总剂量为40mg / kg)显著降低BUN,血清肌酐和血清NGAL。JSH-23导致肾脏中ATN评分和MPO活性显著降低,但不是肾小管细胞凋亡评分。JSH-23也显著降低IL-1,IL-6,CXCL1和TNF-α。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同,这是由实验系统的误差引起的,属于正常现象。 |
References: [1] Shin HM, Kim MH, Kim BH, Jung SH, Kim YS, Park HJ, Hong JT, Min KR, Kim Y. Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. FEBS Lett. 2004 Jul 30;571(1-3):50-4. [2] Ozkok A1, Ravichandran K1, Wang Q1, et al. NF-κB transcriptional inhibition ameliorates cisplatin-induced acute kidney injury (AKI). Toxicol Lett. 2016 Jan 5;240(1):105-13. |
描述 | JSH-23是NF-κB转录活性的抑制剂,IC50值为7.1 μM。 | |||||
靶点 | NF-κB | |||||
IC50 | 7.1 μM |
质量控制和MSDS
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