Tautomycetin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Tautomycetin is a potent, selective inhibitor of PP1, with IC50 values being 1.6 nM and 62 nM for the PP1 and PP2A subtypes, respectively. PP1, one of four major protein serine/threonine phosphatases (PPs), plays an important role in regulation of many cellular events such as transcription, translation, cell division, muscle contraction, glycogen synthesis, and neuronal signaling. Compared with over 40 species of natural phosphatase inhibitors reported, tautomycetin is the most specific PP1 inhibitor, which can be served as a novel powerful tool to elucidate the physiological roles of PP1 in a variety of biological events.
Reference:
1. Mitsuhashi S, Matsuura N, Ubukata M, et al. Tautomycetin is a novel and specific inhibitor of serine/threonine protein phosphatase type 1, PP1. Biochemical and Biophysical Research Communications, 2001, 287(2): 328-331.
| Physical Appearance | Colourless liquid |
| Storage | Desiccate at -20°C |
| M.Wt | 606.75 |
| Cas No. | 119757-73-2 |
| Formula | C33H50O10 |
| Solubility | Soluble in DMSO (supplied pre-dissolved in DMSO, 10mg/ml) |
| Chemical Name | (S)-(2S,3S,4R,7S,8R,11R,13R,Z)-17-ethyl-4,8-dihydroxy-3,7,11,13-tetramethyl-6,15-dioxononadeca-16,18-dien-2-yl 3-hydroxy-3-(4-methyl-2,5-dioxo-2,5-dihydrofuran-3-yl)propanoate |
| SDF | Download SDF |
| Canonical SMILES | O[C@@H]([C@H](C)C(C[C@H]([C@H](C)[C@H](C)OC(C[C@@H](C1=C(C)C(OC1=O)=O)O)=O)O)=O)CC[C@@H](C)C[C@@H](C)CC(/C=C(C=C)/CC)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
人类原代T细胞 |
|
溶解方法 |
在DMSO中的溶解度>10 mM。为了获得更高浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
1 μg/ml，5小时 |
|
应用 |
Tautomycetin具有抑制IL-2分泌从而抑制T细胞增殖的免疫抑制活性。 |
| 动物实验 [2]: | |
|
动物模型 |
异体心脏移植术后的大鼠 |
|
剂量 |
0.05 mg/kg。Tautomycetin溶解于PBS中，以微乳液形式给药30天。 |
|
应用 |
在体内器官移植模型中（异体心脏移植术后的大鼠），Tautomycetin有免疫抑制活性。 |
|
注意事项 |
请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的，属于正常现象。 |
|
References: [1] Jae-Hyuck Shim, et al. Immunosuppressive effects of tautomycetin in vivo and in vitro via T cell-specific apoptosis induction. Proc Natl Acad Sci U S A. 2002 Aug 6; 99(16): 10617–10622. | |
质量控制和MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
化学结构
