SQ 22536
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SQ22536 is an inhibitor of adenylyl cyclase with an IC50 value of 13 μM, it can inhibit prostaglandin E1-stimulated increase in cAMP in intact platelets[1].
In HMC-1 cells and hCBMCs, SQ22536 at a concentrationof 10 mM completely abrogated protein kinase A activity inboth cells. SQ22536 alone at the concentra-tions used did not alter the basal level of protein kinase Aactivity and did not affect cell viability[2].
In KK/Ta-Akita mice, SQ22536 (2 mg/kg, per day) is able to inhibit the renal protection of liraglutide. The combination of liraglutide and SQ22536 can eliminate the improvement of liraglutide on the pathological damage of glomerular tissue. After SQ22536 treatment, renal cAMP does not increase[3].
References:
[1]. Haslam R J, Davidson M M L, and Desjardins J V. Inhibition of adenylate cyclase by adenosine analogues in preparations of broken and intact human platelets. Biochemistry Journal, 1978, 176: 83-95.
[2]. Cao J, Cetrulo C L, Theoharides T C. Corticotropin-releasing hormone induces vascular endothelial growth factor release from human mast cells via the cAMP/protein kinase A/p38 mitogen-activated protein kinase pathway, 2006, 69(3): 998-1006.
[3]. Fujita H, Morii T, Fujishima H, et al. The protective roles of GLP-1R signaling in diabetic nephropathy: possible mechanism and therapeutic potential. Kidney International, 2014, 85(3): 579-589.
Storage | Store at -20°C |
M.Wt | 205.22 |
Cas No. | 17318-31-9 |
Formula | C9H11N5O |
Solubility | insoluble in EtOH; ≥20.5 mg/mL in DMSO; ≥26.4 mg/mL in H2O |
Chemical Name | (R)-9-(tetrahydrofuran-2-yl)-9H-purin-6-amine |
SDF | Download SDF |
Canonical SMILES | NC(N=CN=C12)=C2N=CN1[C@@H]3OCCC3 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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