NSC 66811
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NSC 66811是一种小分子的鼠双微体2（MDM2）-p53相互作用的抑制剂，Ki值为120 nM[1]。
p53的N端和MDM2的深疏水裂缝可在p53和MDM2之间形成一个短螺旋链。NSC 66811可与这一裂缝的活性位点结合。NSC 66811具有高结合亲和力，Ki值为120 nM，比天然p53多肽强56倍。NSC 66811的立位异构体模仿p53的三个关键残基（Phe19、Trp26和Leu26），介导与MDM2的相互作用。在具有野生型p53的HCT-116人类结肠癌细胞系中，NSC 66811可减少p53、MDM2和p21cip1/waf蛋白的积累。但是，NSC 66811在同基因的HCT-116 p53 /细胞系中对p53、MDM2和p21cip1/waf蛋白水平并无影响[1]。
参考文献：
[1] Lu Y, Nikolovska-Coleska Z, Fang X, Gao W, Shangary S, Qiu S, Qin D, Wang S. Discovery of a nanomolar inhibitor of the human murine double minute 2 (MDM2)-p53 interaction through an integrated, virtual database screening strategy. J Med Chem. 2006 Jun 29;49(13):3759-62.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 340.42 |
| Cas No. | 6964-62-1 |
| Formula | C23H20N2O |
| Solubility | ≥34 mg/mL in DMSO; insoluble in H2O; ≥3.82 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | 7-[anilino(phenyl)methyl]-2-methylquinolin-8-ol |
| SDF | Download SDF |
| Canonical SMILES | CC1=NC2=C(C=C1)C=CC(=C2O)C(C3=CC=CC=C3)NC4=CC=CC=C4 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
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质量控制和MSDS
- 批次:
化学结构
