NPS-2143
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NPS-2143是新型的CaSR(钙离子敏感受体)选择性拮抗剂,对[Ca2+]o的EC50值为4.27 mM[1].
据报道,Calcilytics NPS-2143减弱CaSR对[Ca2+]o的敏感度,从而在体外减弱信号转导并在体内促进PTH分泌.NPS-2143对野生型CaSR以及突变型CaSR(T151R\P221L\E767Q\G830S和A844T)的EC50值分别为4.27\1.56\1.61\2.46\2.07和3.15 mM.此外,在表达CaSR的HEK 293T细胞中,NPS-2143呈浓度依赖性地抑制[Ca2+]o诱导的细胞内钙信号传导[1].
参考文献：
[1] Letz S1, Rus R, Haag C, Drr HG, Schnabel D, Mhlig M, Schulze E, Frank-Raue K, Raue F, Mayr B, Schfl C. Novel activating mutations of the calcium-sensing receptor: the calcilytic NPS-2143 mitigates excessive signal transduction of mutant receptors. J Clin Endocrinol Metab. 2010 Oct;95(10):E229-33.
| Storage | Store at -20°C |
| M.Wt | 408.93 |
| Cas No. | 284035-33-2 |
| Formula | C24H25ClN2O2 |
| Solubility | ≥40.9 mg/mL in DMSO; insoluble in H2O; ≥28.35 mg/mL in EtOH |
| Chemical Name | 2-chloro-6-[(2R)-2-hydroxy-3-[(2-methyl-1-naphthalen-2-ylpropan-2-yl)amino]propoxy]benzonitrile |
| SDF | Download SDF |
| Canonical SMILES | CC(C)(CC1=CC2=CC=CC=C2C=C1)NCC(COC3=C(C(=CC=C3)Cl)C#N)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | NPS-2143是新型的\有效的和选择性的钙离子受体拮抗剂,其IC50值为43 nM. | |||||
| 靶点 | Ca(2+) receptor | |||||
| IC50 | 43 nM | |||||
质量控制和MSDS
- 批次:
化学结构
