MHY1485
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
MHY1485是mTOR的强效激活剂,抑制自噬,并抑制自噬体与溶酶体之间的融合[1].
mTOR(雷帕霉素机械靶点)是一种丝氨酸/苏氨酸激酶,参与调节细胞代谢\生长和存活.它介导细胞对压力\生长因子和激素等的应答,mTOR的抑制强烈诱导自噬反应.mTOR信号在促进卵泡发育中也具有重要作用.
在Ac2F大鼠肝细胞中,MHY1485治疗抑制基础自噬通量,对细胞饥饿诱导的自噬也具有抑制效果.MHY1485以剂量和时间依赖性的方式引起LC3II的积累和自噬体的扩大[1].
使用MHY1485培养幼年小鼠卵巢4天,增加卵巢外植体的重量和卵泡发育.MHY1485预孵育这些卵巢2天,随后将基因移植到切除卵巢的成年肾内5天,移植物重量明显增加,并促进卵泡发育[2].
参考文献：
1, Choi YJ, Park YJ, Park JY et al. Inhibitory effect of mTOR activator MHY1485 onautophagy: suppression of lysosomal fusion. PLoS One. 2012;7(8):e43418. doi:10.1371/journal.pone.0043418. Epub 2012 Aug 22. Erratum in: PLoS One. 2013;8(1).
2, Cheng Y, Kim J, Li XX, Hsueh AJ. Promotion of ovarian follicle growth following mTOR activation: synergistic effects of AKT stimulators. PLoS One. 2015 Feb 24;10(2):e0117769.
- 1. Bo Yang, Yan Zhou, et al. "ω-6 Polyunsaturated fatty acids (linoleic acid) activate both autophagy and antioxidation in a synergistic feedback loop via TOR-dependent and TOR-independent signaling pathways." Cell Death Dis. 2020 Jul 30;11(7):607. PMID:32732901
- 2. Huang Z, Mou T, et al. "Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB." Cell Death Dis. 2020;11(6):455. PMID:32532961
- 3. Gao L, Lv G, et al. "Glycochenodeoxycholate promotes hepatocellular carcinoma invasion and migration by AMPK/mTOR dependent autophagy activation." Cancer Lett. 2019 Jul 10;454:215-223. PMID:30980867
- 4. Wang J, Yang C, et al. "T-2 Toxin Exposure Induces Apoptosis in TM3 Cells by Inhibiting Mammalian Target of Rapamycin/Serine/Threonine Protein Kinase(mTORC2/AKT) to Promote Ca(2+)Production." Int J Mol Sci. 2018 Oct 27;19(11). pii: E3360. PMID:30373220
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 387.39 |
| Cas No. | 326914-06-1 |
| Formula | C17H21N7O4 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥19.35 mg/mL in DMSO |
| Chemical Name | 4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine |
| SDF | Download SDF |
| Canonical SMILES | [O-][N+](C1=CC=C(NC2=NC(N3CCOCC3)=NC(N4CCOCC4)=N2)C=C1)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
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细胞系 |
Ac2F细胞 |
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制备方法 |
在DMSO中的溶解度大于19.4 mg/mL。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
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反应条件 |
2 μM；6小时 |
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实验结果 |
使用AdGFP-LC3转染Ac2F细胞，并采用LysoTracker进行染色（用于显示溶酶体）。在饥饿状态下，MHY1485降低LC3和溶酶体的重叠信号。因此，MHY1485抑制饥饿诱导的自噬体与溶酶体的融合。 |
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References: [1]. Choi YJ, Park YJ, Park JY, Jeong HO, Kim DH, Ha YM, Kim JM, Song YM, Heo HS, Yu BP, Chun P, Moon HR, Chung HY. Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion. PLoS One. 2012;7(8):e43418. |
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质量控制和MSDS
- 批次:
化学结构
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