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MHY1485

现货
Catalog No.
B5853
mTOR激活剂,自噬抑制剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 500.00
现货
10mg
¥ 700.00
现货
50mg
¥ 2,450.00
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200mg
¥ 5,250.00
现货

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Background

MHY1485 is a potent mTOR activator that inhibits autophagy with suppression of fusion between autophagosomes and lysosomes. [1]

mTOR (mechanistic target of rapamycin) is a serine/threonine kinase that regulates cellular metabolism, growth ad survival. It mediates the cellular response to stress, growth factor and hormone etc. Inhibition of mTOR strongly induced autophagy. mTOR signaling also plays a role in the promotion of ovarian follicle development.

In Ac2F rat hepatocytes, MHY1485 treatment suppresses the basal autophagic flux, and it also shows inhibitory effect under cell starvation which is an inducer of autophagy. The effects of MHY1485 dose-and time- dependently cause accumulation of LC3II and enlargement of the autophagosomes. [1]

Ovaries of juvenile mice cultured with MHY1485 for 4 days increases ovarian explant weights and follicle development. 2 days pre-incubation of these ovaries with MHY1485 followed by allo-grafting into kidney capsules of adult ovariectomized hosts for 5 days, shows apparent increases in graft weights and promotion of follicle development. [2]

References:
1, Choi YJ, Park YJ, Park JY et al.  Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion.  PLoS One. 2012;7(8):e43418. doi:10. 1371/journal.pone.0043418. Epub 2012 Aug 22. Erratum in: PLoS One. 2013;8(1).
2, Cheng Y, Kim J, Li XX, Hsueh AJ.  Promotion of ovarian follicle growth following mTOR activation: synergistic effects of AKT stimulators.  PLoS One. 2015 Feb 24;10(2):e0117769. 

文献引用

1. Gao L, Lv G, et al. "Glycochenodeoxycholate promotes hepatocellular carcinoma invasion and migration by AMPK/mTOR dependent autophagy activation." Cancer Lett. 2019 Jul 10;454:215-223. PMID:30980867
2. Wang J, Yang C, et al. "T-2 Toxin Exposure Induces Apoptosis in TM3 Cells by Inhibiting Mammalian Target of Rapamycin/Serine/Threonine Protein Kinase(mTORC2/AKT) to Promote Ca(2+)Production." Int J Mol Sci. 2018 Oct 27;19(11). pii: E3360. PMID:30373220

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt387.39
Cas No.326914-06-1
FormulaC17H21N7O4
Solubility≥19.35mg/mL in DMSO
Chemical Name4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine
SDFDownload SDF
Canonical SMILES[O-][N+](C1=CC=C(NC2=NC(N3CCOCC3)=NC(N4CCOCC4)=N2)C=C1)=O
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验 [1]:

细胞系

Ac2F细胞

制备方法

在DMSO中的溶解度大于19.4 mg/mL。若配制更高浓度的溶液,一般步骤如下:请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。

反应条件

2 μM;6小时

实验结果

使用AdGFP-LC3转染Ac2F细胞,并采用LysoTracker进行染色(用于显示溶酶体)。在饥饿状态下,MHY1485降低LC3和溶酶体的重叠信号。因此,MHY1485抑制饥饿诱导的自噬体与溶酶体的融合。

References:

[1]. Choi YJ, Park YJ, Park JY, Jeong HO, Kim DH, Ha YM, Kim JM, Song YM, Heo HS, Yu BP, Chun P, Moon HR, Chung HY. Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion. PLoS One. 2012;7(8):e43418.

质量控制

化学结构

MHY1485

相关生物数据

MHY1485

相关生物数据

MHY1485