Leflunomide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Leflunomide（来氟米特）是芳香烃受体（AHR）的激动剂[1]。Leflunomide是可以口服的免疫调节剂。A771726（teriflunomide）是由leflunomide形成的非酶促活性药[2]。
研究表明，Leflunomide可以增加外周血和干细胞亚群的Tregs水平，增加这些细胞向受损的肾的迁移。在缺血再灌注肾中，Leflunomide可以增加细胞IL-10的表达，减少IL-17和IL-23的表达[1]。
在雄性脓毒症Wistar大白鼠中，Leflunomide显著降低脂质过氧化。同时，leflunomide成功地抑制肠中蛋白质羰基和NO的增加[2]。在人MTC-TT细胞中，Leflunomide以剂量和时间依赖的方式减少细胞增殖，抑制肿瘤标志物表达以及下调神经内分泌标记物ASCL1的蛋白表达[3]。
参考文献：
[1] Baban B1,Liu JY,Mozaffari MS. Aryl hydrocarbon receptor agonist, leflunomide, protects the ischemic-reperfused kidney: role of Tregs and stem cells. Am J Physiol Regul Integr Comp Physiol.2012 Dec;303(11):R1136-46.
[2] Ozturk E1,Surucu M2,Karaman A3,SamdancE4,Fadillioglu E5. Protective effect of leflunomideagainstoxidative intestinal injury in a rodent model of sepsis. J Surg Res.2014 Apr;187(2):610-5.
[3] Alhefdhi A1,Burke JF,Redlich A,Kunnimalaiyaan M,Chen H. Leflunomidesuppresses growth in human medullary thyroid cancer cells. J Surg Res.2013 Nov;185(1):212-6.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 270.21 |
| Cas No. | 75706-12-6 |
| Formula | C12H9F3N2O2 |
| Solubility | ≥83.33 mg/mL in DMSO; insoluble in H2O; ≥18.57 mg/mL in EtOH |
| Chemical Name | 5-methyl-N-[4-(trifluoromethyl)phenyl]-1,2-oxazole-4-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C=NO1)C(=O)NC2=CC=C(C=C2)C(F)(F)F |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
Human medullary thyroid cancer cell line TT (MTC-TT cells) |
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Reaction Conditions |
75 ~ 150 μmol/L leflunomide for 2, 4, or 6 d incubation |
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Applications |
Leflunomide treatment down-regulated neuroendocrine markers achaete-scute complex-like 1 (ASCL1) and chromogranin A. Moreover, leflunomide significantly inhibited the growth of MTC-TT cells in a dose- and time- dependent manner. |
| Animal experiment:[2] | |
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Animal models |
Male C57BL/6 mice (10 ~ 12 wk of age) |
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Dosage form |
40 mg/kg Injected intraperitoneally16 h prior to the induction of renal ischemic reperfusion insult |
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Applications |
Leflunomide treatment increased regulatory T cells and IL-10-positive cells but reduced IL-17- and IL-23-expressing cells in both the peripheral blood and kidney cells, indicative of down-regulation of inflammatory responses. Leflunomide treatment also increased mobilization of stems cells subsets in the peripheral blood and promoted their recruitment into the ischemic-reperfused kidney. Eventually, leflunomide treatment was able to preserve mitochondrial membrane potential as well as decrease apoptosis and necrosis of renal cells. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Alhefdhi A, Burke JF, Redlich A, et al. Leflunomide suppresses growth in human medullary thyroid cancer cells. Journal of Surgical Research, 2013, 185(1): 212-216. 2. Baban B, Liu JY, Mozaffari MS. Aryl hydrocarbon receptor agonist, leflunomide, protects the ischemic-reperfused kidney: role of Tregs and stem cells. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 2012, 303(11): R1136-1146. |
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质量控制和MSDS
- 批次:
化学结构
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