L-NAME hydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
L-NAME Hydrochloride is an inhibitor of nitric oxide synthase (NOS) with IC50 value of 70 μM [1].
NOS is an important enzyme involved in production of NO. NO, the smallest signaling molecule known, controls servoregulatory functions such as neurotransmission or vascular tone, regulates gene transcription and mRNA translation, as well as produces post-translational modifications of proteins [2].
At concentrations of 10-9 ~ 10-3 M, L-NAME Hydrochloride inhibited NOS purified from rat brains in a dose dependent manner, with IC50 value of 70 μM [1]. In another study, L-NAME Hydrochloride at 0.1 ~ 100 mM caused concentration-dependent inhibition of the Ca2+-dependent endothelial NO synthase from porcine aortae. L-NAME Hydrochloride also caused an endothelium-dependent contraction and an inhibition of the endothelium-dependent relaxation induced by acetylcholine (ACh) in aortic rings [3].
In anaesthetized rats, L-NAME Hydrochloride (0.03 ~ 300 mg/kg, i.v.) induced a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME Hydrochloride (100 mg/kg, i.v.) significantly inhibited the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME Hydrochloride could be reversed by L-arginine (30 ~ 100 mg/kg, i.v.) in a dose-dependent manner [3].
References:
[1]. Pfeiffer S, Leopold E, Schmidt K, et al. Inhibition of nitric oxide synthesis by NG-nitro-L-arginine methyl ester (L-NAME): requirement for bioactivation to the free acid, NG-nitro-L-arginine. British Journal of Pharmacology, 1996, 118(6): 1433-1440.
[2]. Förstermann U, Sessa W C. Nitric oxide synthases: regulation and function. European Heart Journal, 2012, 33(7): 829-837.
[3]. Rees D D, Palmer R M, Schulz R, et al. Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo. British Journal of Pharmacology, 1990, 101(3): 746-752.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 269.7 |
| Cas No. | 51298-62-5 |
| Formula | C7H15N5O4·HCl |
| Synonyms | L-NAME,L-NAME HCl |
| Solubility | ≥27 mg/mL in H2O; insoluble in EtOH; ≥23 mg/mL in DMSO |
| Chemical Name | methyl (2S)-2-amino-5-[[amino(nitramido)methylidene]amino]pentanoate;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | COC(=O)C(CCCN=C(N)N[N+](=O)[O-])N.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
Bovine retinal endothelial cells (BRECs) and rat retinal müller cell line rMC-1 |
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Reaction Conditions |
1 mM L-NAME for 5 d incubation |
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Applications |
L-NAME inhibited production of NO and prostaglandin E2 as well as expression of iNOS and COX-2 in rMC-1 cells exposed to 25 mM glucose. Moreover, L-NAME also reduced BREC and rMC-1 cell death in the high glucose condition. |
| Animal experiment:[2] | |
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Animal models |
Male Wistar rats, 200 ~ 300 g |
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Dosage form |
0.03 ~ 300 mg/kg Intravenous administration (i.v.) |
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Applications |
L-NAME (0.03 ~ 300 mg/kg, i.v.) induced a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. Furthermore, L-NAME (100 mg/kg, i.v.) inhibited significantly the hypotensive responses to acetylcholine and bradykinin. The increases in blood pressure and bradycardia produced by L-NAME were reversed by L-arginine (30 ~ 100 mg/kg, i.v.) in a dose-dependent manner. Therefore, L-NAME could be used to study the important role of NO synthesis in the maintenance of vascular tone and blood pressure. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Du Y, Sarthy VP, Kern TS. Interaction between NO and COX pathways in retinal cells exposed to elevated glucose and retina of diabetic rats. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2004, 287(4): R735-741. 2. Rees DD, Palmer RM, Schulz R, et al. Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo. British Journal of Pharmacology, 1990, 101(3): 746-752. |
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| Description | L-NAME hydrochloride is a NO synthase inhibitor. | |||||
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| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
