Histone-H2A-(107-122)-Ac-OH
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
组蛋白H2A-(107-122)-Ac-OH的化学式为C81H140N20O23，氨基酸序列为Ac-Gly-Val-Leu-Pro-Asn-Ile-Gln-Ala-Val-Leu-Leu-Pro-Lys-Lys-Thr-Glu-OH，分子量为 1762.1。在真核细胞中主要有五种组蛋白，组蛋白H2A是其中一种，参与真核细胞染色质的结构。组蛋白是将DNA包装成核小体的蛋白(1)。组蛋白负责维持核小体的形状和结构。一个染色质分子中每100个DNA碱基对至少含有一个核心组蛋白。迄今为止，鉴定到五种组蛋白，分别为H1 / H5、H2A、H2B、H3和H4(2)。H2A对将DNA包裹成染色质非常重要。H2A包裹DNA分子进入染色质，包装过程可以影响基因表达。H2A与DNA修饰和表观遗传学相关。H2A在确定染色质的整体结构中发挥主要作用，还参与调节基因表达(3)。H2A 介导的DNA修饰发生在细胞核中，核转运蛋白和输入蛋白对于H2A蛋白的转运有重要作用。最近的研究还表明，核小体装配蛋白1用于将H2A运输到核中，以便它可以包裹DNA。组蛋白变体H2A.Z中可以观察到组蛋白的其他功能，这种变异与基因激活、沉默和抑制反义RNA有关。此外，在人类和酵母细胞中研究H2A.Z发现，H2A.Z可用于促进RNA聚合酶II招募(4)。
Figure1 the structures of Histone-H2A
Figure 2 Schematics of Histone-H2A abundance and chromatin composition
参考文献：
1. Youngson, Robert M. (2006).Collins Dictionary of Human Biology.Glasgow: HarperCollins.
2. Khorasanizadeh, Sepideh. 2004. The Nucleosome: From Genomic Organization to Genomic Regulation. Department of Biochemistry and Molecular Genetics,UniversityofVirginia HealthSystem. Cellular Biology. Volume 116, Issue 2, , Pages 259–272.
3. Marino-Ramirez L, Jordan IK, Landsman D. Multiple independent evolutionary solutions to core histone gene regulation. Genome Biol. 2006;7:R12
4. Marino-Ramirez L, Levine KM, Morales M, Zhang S, Moreland. RT, Baxevanis AD, Landsman D (2011) The histone database: an integrated resource for histones and histone fold-containing proteins. Database (Oxford) bar048
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 1762.1 |
| Formula | C81H140N20O23 |
| Synonyms | Ac-Gly-Val-Leu-Pro-Asn-Ile-Gln-Ala-Val-Leu-Leu-Pro-Lys-Lys-Thr-Glu-OH |
| Solubility | ≥176.2 mg/mL in DMSO; insoluble in EtOH; ≥23.3 mg/mL in H2O |
| SDF | Download SDF |
| Canonical SMILES | CC(NCC(NC(C(C)C)C(NC(CC(C)C)C(N1C(C(NC(CC(N)=O)C(NC(C(CC)C)C(NC(CCC(N)=O)C(NC(C)C(NC(C(NC(CC(C)C)C(NC(CC(C)C)C(N2C(C(NC(CCCCN)C(NC(CCCCN)C(NC(C(C)O)C(NC(C(O)=O)CCC(O)=O)=O)=O)=O)=O)CCC2)=O)=O)=O)C(C)C)=O)=O)=O)=O)=O)CCC1)=O)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
