GSK J1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
GSK J1是一种有效的和选择性的组蛋白H3赖氨酸27（H3K27）去甲基化酶JMJD3的抑制剂，半数最大有效抑制浓度IC50值为60 nM。JMJD3是一种Fe2+和α-酮戊二酸依赖的加氧酶，参与包括炎症反应在内的多种生理学功能[1]。
然而，GSK J1的细胞可透过性有限，因其具有高极性的羧基基团，其在GSK J1与JMJD3的结合过程中具有重要作用。为了提高细胞可透过性，GSK J1被酯化以弱化其极性，使之成为可快速水解为GSK J1的乙酯衍生物[1]。
参考文献：
[1] Kruidenier L, Chung CW, Cheng Z, Liddle J, Che K, Joberty G, Bantscheff M, Bountra C, Bridges A, Diallo H, Eberhard D, Hutchinson S, Jones E, Katso R, Leveridge M, Mander PK, Mosley J, Ramirez-Molina C, Rowland P, Schofield CJ, Sheppard RJ, Smith JE, Swales C, Tanner R, Thomas P, Tumber A, Drewes G, Oppermann U, Patel DJ, Lee K, Wilson DM. A selective jumonji H3K27 demethylase inhibitor modulates the proinflammatory macrophage response. Nature. 2012 Aug 16;488(7411):404-8. doi: 10.1038/nature11262.
| Storage | Store at -20°C |
| M.Wt | 389.45 |
| Cas No. | 1373422-53-7 |
| Formula | C22H23N5O2 |
| Solubility | ≥38.9 mg/mL in DMSO; insoluble in H2O; ≥51.2 mg/mL in EtOH with ultrasonic |
| Chemical Name | 3-[[2-pyridin-2-yl-6-(1,2,4,5-tetrahydro-3-benzazepin-3-yl)pyrimidin-4-yl]amino]propanoic acid |
| SDF | Download SDF |
| Canonical SMILES | C1CN(CCC2=CC=CC=C21)C3=CC(=NC(=N3)C4=CC=CC=N4)NCCC(=O)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
