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Geneticin, G-418 Sulfate

现货
Catalog No.
A2513
氨基糖甙抗生素
组合的产品项目
规格价格库存 数量
1g
¥ 700.00
现货
5g
¥ 2,100.00
现货

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Background

G-418 is an elongation inhibitor of 80 S ribosomes [1].

G418 is an aminoglycoside antibiotic, it has a wide spectrum of activity and is active against bacteria, yeasts, fungi, algae, plant and even animal cells. G418 is usually used as a cloning selection in pro- and eukaryotes at concentrations from 1 to 300 g/ml. In the studies of antibiotic resistance in clinical isolates of bactenia, it is found that all strains carrying 3'-O-aminoglycoside phosphotransferases are resistant to G418. Among these phosphotransferases, type I and type II are encoded by the transposable elements TnS and Tn601, respectively [2].

Apart from the selector function, G418 is also found to have antiviral activity against bovine viral diarrhea virus (BVDV). G418 prevents the cytopathic effect (CPE) resulting from DENV-2 infection of BHK cells, in a dose-dependent manner with an EC50 value of 3±0.4 g/ml [3].

References:
[1] Bar-Nun S, Shneyour Y, Beckmann JS. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 1983, 741 (1): 123-7.
[2] Julian Davies and Antonio Jimenez. A new selective agent for eukaryotic cloning vectors. Am. J. Trop. Med. Hyg. 1980, 29 (5): 1089-1092.
[3] Xianchao G. Zhang, Peter W. Mason, Edward J. Dubovi, Xiaodong Xu, Nigel Bourne, Randall W. Renshaw, Timothy M. Block, and Alexander V. Birk. Antiviral activity of Geneticin against DENGUE virus. Antiviral Res. 2009 July, 83(1): 21–27.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt692.71
Cas No.108321-42-2
FormulaC20H40N4O10.2H2SO4
SynonymsAntibiotic G418
Solubility<1.34mg/mL in DMSO; ≥240.4 mg/ml in H2O
Chemical Name2-[4,6-diamino-3-[3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
SDFDownload SDF
Canonical SMILESCC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验 [1, 2]:

细胞系

DENV-2转染的BHK细胞

溶解方法

该化合物可溶于DMSO。若获取更高浓度的溶液,可在37℃下孵育10分钟,随后在超声波浴中摇匀。-20℃以下可储存数月。

反应条件

1–300 μg/ml,120 h

应用

Geneticin(1-300 μg/ml)抑制多种原核及真核生物。Geneticin降低BHK细胞的活力,CC50值为165 ± 5 μg/ml。Geneticin预防DENV诱导的CPE,增加感染细胞的活力,EC50值大约为3 ± 0.4 μg/ml。Geneticin在72hpi后抑制DENV-2的病毒滴度的产量,EC50值为2 ± 0.1 μg/ml,EC90值约为20 ± 2 μg/ml。Geneticin抑制病毒产生的斑块数量,并显著减少剩余斑块的大小。

注意事项

由于实验环境的不同,实际溶解度可能与理论值略有不同,请测试室内所有化合物的溶解度。

References:

[1] Bar-Nun S, Shneyour Y, Beckmann JS. G-418, an elongation inhibitor of 80 S ribosomes. Biochim Biophys Acta. 1983, 741 (1): 123-7.

[2] Julian Davies and Antonio Jimenez. A new selective agent for eukaryotic cloning vectors. Am. J. Trop. Med. Hyg. 1980, 29 (5): 1089-1092. [3] Xianchao G. Zhang, Peter W. Mason, Edward J. Dubovi, Xiaodong Xu, Nigel Bourne, Randall W. Renshaw, Timothy M. Block, and Alexander V. Birk. Antiviral activity of Geneticin against DENGUE virus. Antiviral Res. 2009 July, 83(1): 21–27.

生物活性

Description G-418是一种氨基糖甙抗生素。
靶点 80 S ribosomes          
IC50            

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