coagulation factor II (thrombin) B chain fragment [Homo sapiens]
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
凝血酶的氨基酸序列为H2N-Lys-Pro-Val-Ala-Phe-Ser-Asp-Tyr-Ile-His-Pro-Val-Cys-Leu-Pro-Asp-Arg-OH,是一种由人F2基因编码的“胰蛋白酶样”丝氨酸蛋白酶[1]。活化的Factor X (Xa) 在凝血酶原两个位点进行酶切,将其裂解为凝血酶。凝血酶反过来作为一种丝氨酸蛋白酶,将可溶性纤维蛋白原转变成不溶性的纤维蛋白。凝血酶也可催化许多其他凝血相关反应。
在凝血过程中,凝血酶发挥作用,将Factor XI 转变成Xia、VIII 转变成VIIIa、V 转变成Va以及fibrinogen转变成 fibrin。作为凝血级联活动的一部分,凝血酶可以激活血小板细胞膜上的蛋白酶激活受体,促进血小板激活和聚集。
凝血酶是一种有效的血管收缩剂和促细胞分裂剂,在蛛网膜下腔出血后的血管痉挛中发挥重要作用。血流从脑动脉周围的破裂脑动脉瘤栓塞流出,释放凝血酶,可诱导血管急性和长期的变窄,最终可能会导致脑缺血和梗死(中风)。
除了在血栓形成的动态过程中的关键作用,凝血酶还具有明显的促炎性质,这可能会影响动脉粥样硬化的发生和发展[2,3]。
Fig 1. The structure of human thrombin in complex with the inhibitor hirudin.
Fig 2. Role of thrombin in the blood coagulation cascade
参考文献:
1. Royle NJ, Irwin DM, Koschinsky ML, MacGillivray RT, Hamerton JL (May 1987). "Human genes encoding prothrombin and ceruloplasmin map to 11p11-q12 and 3q21-24, respectively". Somat. Cell Mol. Genet. 13 (3): 285–92.
2. Borissoff JI, Spronk HM, Heeneman S, ten Cate H. Is thrombin a key player in the 'coagulation-atherogenesis' maze? Cardiovasc Res. 2009;82(3):392-403.
3. Borissoff JI, Heeneman S, Kilinc E, Kassak P, Van Oerle R, Winckers K, Govers-Riemslag JW, Hamulyak K, Hackeng TM, Daemen MJ, ten Cate H, Spronk HM. Early atherosclerosis exhibits an enhanced procoagulant state. Circulation. 2010;122(8):821-830. PMID20697022.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1957.26 |
Formula | C90H137N23O24S |
Synonyms | H2N-Lys-Pro-Val-Ala-Phe-Ser-Asp-Tyr-Ile-His-Pro-Val-Cys-Leu-Pro-Asp-Arg-OH |
Solubility | insoluble in EtOH; ≥17.6 mg/mL in H2O; ≥195.7 mg/mL in DMSO |
SDF | Download SDF |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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