Chlorothricin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 173, 500, 260, and 120 μM for pyruvate carboxylases from Bacillus, Azotobacter, rat, and chicken, respectively.
Chlorothricin is a macrolide-type antibiotic.
Macrolides, a class of natural products belonging to the polyketide class of natural products, consist of a large macrocyclic lactone ring. The lactone rings are oftem 14-, 15-, or 16-membered. Some macrolides have been reported to have antibiotic or antifungal activity and are widely used as pharmaceutical drugs.
In vitro: In a previous study, chlorothricin was found to inhibit the reaction catalyzed by pyruvate carboxylase from Bacillus stearothermophilus. Moreover, with steady-state kinetic measurements, inhibition of the overall reaction was found to be competitive with the allosteric activator of this enzyme, CoASAc, and non-competitive with respect to both substrates of MgATP and pyruvate. These findings strongly indicated that the site 1conformation of pyruvate carboxylase responsible for the regulation of the overall enzyme activity could be influenced by chlorothricin and CoASAc in an antagonistic manner [1].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Schindler PW, Zhner H. Mode of action of the macrolide-type antibiotic, chlorothricin. Kinetic study of the inhibition of pyruvate carboxylase from Bacillus stearothermophilus. Eur J Biochem. 1973 Nov 15;39(2):591-600.
| Physical Appearance | A white solid |
| Storage | Store at -20°C |
| M.Wt | 955.5 |
| Cas No. | 34707-92-1 |
| Formula | C50H63ClO16 |
| Synonyms | Antibiotic K 818A |
| Solubility | Soluble in ethanol;Soluble in methanol;Soluble in DMSO;Soluble in dimethyl formamide |
| Chemical Name | (4S,4aS,6aR,11E,12aR,15R,16aS,21aR,21bR)-4-[[4-O-[3-O-(3-chloro-6-methoxy-2-methylbenzoyl)-2,6-dideoxy-β-D-arabino-hexopyranosyl]-2,6-dideoxy-β-D-arabino-hexopyranosyl]oxy]-1,2,3,4,4a,6a,7,8,9,10,12a,15,16,21,21a,21b-hexadecahydro-22-hydroxy-15,21a-dimeth |
| SDF | Download SDF |
| Canonical SMILES | OC([C@]1([C@@]([H])(C=C([C@@H](C1)C)C(O)=O)/C=C\CCCC[C@]2([H])C=C[C@@]3([H])[C@](CCC[C@@H]3O[C@@]([H])(C[C@H]4O)O[C@@H]([C@H]4O[C@@]([H])(C[C@H]5OC(C6=C(OC)C=CC(Cl)=C6C)=O)O[C@@H]([C@H]5O)C)C)([H])[C@@]27C)OC8=O)=C8OC7=O |
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