W123
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
W123, an analog of FTY720, is a competitive antagonist of sphingosine-1-phosphate (S1P) type 1 receptor (S1P1), which is measured by GTPγS activation, cell migration, ligand-induced receptor internalization, and mitogen-activated protein kinase recruitment.
S1P plays a vital role in the entire human body, involving in mediating skin, vascular endothelial, and immune cells. S1P1, also known as EDG-1, is one of the five high affinity G protein-coupled S1P receptors and mediates lymphoid, endothelial, and neuronal cell responses to S1P.
In vitro: W123 reversed the effects of S1P1 agonists, FTY720, p-FTY720, and SEW2871. Superoxide dismutase and catalase activity level seen in W123-treated groups were reversely improved via antagonizing the S1P receptor induced by W123. W123 reduced phosphorylated levels of PI3K, Akt, and FoxO3a in different ways, indicating that the activation of S1P1 receptor in the context of oxidative stress elicited the phosphorylation/activation of PI3K/Akt and thus the phosphorylation/inactivation of FoxO3a which in turn promoted PC12 cell survival [1].
In vivo: Up to now, in vivo study of W123 is still in the development stage.
Reference:
[1]. Safarian, F., Khallaghi, B., Ahmadiani, A., & Dargahi, L. Activation of S1P1 Receptor Regulates PI3K/Akt/FoxO3a Pathway in Response to Oxidative Stress in PC12 Cells. Journal of Molecular Neuroscience. 2014; 56(1): 177-187.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 306.4 |
Cas No. | 1345982-24-2 |
Formula | C17H26N2O3 |
Solubility | ≤2mg/ml in ethanol;15mg/ml in DMSO;25mg/ml in dimethyl formamide |
Chemical Name | 3-(2-(3-hexylphenylamino)-2-oxoethylamino)propanoic acid |
SDF | Download SDF |
Canonical SMILES | OC(=O)CCNCC(=O)Nc1cccc(CC2CCCCC2)c1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |