FTY720 Phosphate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
FTY720 is sphingosine-1-phosphate (S1P) receptors agonist [1]. FTY720 is a novel immunomodulatory agent derived from ISP-1 (myriocin), a fungal metabolite that exists in traditional Chinese herbal medicine. In higher eukaryotes, S1P is the ligand for five G-protein-coupled receptors. These S1P receptors are differentially expressed and regulate vascular maturation, angiogenesis, cardiac development and immunity. S1P receptors are important for directed cell movement [2].
In vitro: The phosphorylated FTY720 acted as agonist for a family of G protein-coupled receptors in vitro. FTY720 inhibited sphingosine-1-phosphate lyase activity [3]. FTY720 phosphate acted as a potent agonist at sphingosine-1-phosphate (S1P) receptors S1P1, S1P3, S1P4, and S1P5 with IC50 values of 0.2-6 nM [3].
In vivo: In a variety of transplant and autoimmune models, FTY720 was efficacious without inducing a generalized immunosuppressed state. FTY720 was effective in human kidney transplantation. FTY720 elicited lymphopenia resulting from a reversible redistribution of lymphocytes from circulation to secondary lymphoid tissues by unknown mechanisms. In a rodent model of multiple sclerosis, the phosphorylated FTY720 compound was a potent agonist at four s S1P receptors. Phosphorylation of FTY720 acted through sphingosine 1-phosphate signaling pathways to modulate chemotactic responses and lymphocyte trafficking [1]. FTY720 was phosphorylated in vivo. In mice, treatment with FTY720 inhibited tissue sphingosine-1-phosphate lyase activity, whereas lyase gene and protein expression were not significantly affected [3].
References:
[1] Brinkmann V, Davis M D, Heise C E, et al. The immune modulator FTY720 targets sphingosine 1-phosphate receptors[J]. Journal of Biological Chemistry, 2002, 277(24): 21453-21457.
[2] Spiegel S, Milstien S. Sphingosine-1-phosphate: an enigmatic signalling lipid[J]. Nature reviews Molecular cell biology, 2003, 4(5): 397-407.
[3] Bandhuvula P, Tam Y Y, Oskouian B, et al. The immune modulator FTY720 inhibits sphingosine-1-phosphate lyase activity[J]. Journal of Biological Chemistry, 2005, 280(40): 33697-33700.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 387.5 |
Cas No. | 402615-91-2 |
Formula | C19H34NO5P |
Synonyms | FTY720P |
Solubility | ≤0.5mg/ml in chloroform |
Chemical Name | 2-amino-2[2-(4-octylphenyl)ethyl]-1,3-propanediol, mono dihydrogen phosphate ester |
SDF | Download SDF |
Canonical SMILES | CCCCCCCCC1=CC=C(CCC([NH3+])(COP([O-])(O)=O)CO)C=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |