7,8-dihydro-L-Biopterin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
7,8-dihydro-l-biopterin (BH2), an analogue of the natural cofactor BH4, is a precursor in the synthesis of BH4 [1].
Tetrahydrobiopterin (BH4) is a key redox-active cofactor involved in endothelial isoform of NO synthase (eNOS) catalysis. BH4 is an important determinant of NO-dependent signaling pathways. Oxidation of BH4 has been observed in vascular cells in the setting of the oxidative stress associated with diabetes [1,2].
In cultured aortic endothelial cells, supplementation with BH2 abolished VEGF-induced NO production. DHFR but not GTPCH1 knockdown increased reactive oxygen species (ROS) production. BH2 abolished the increase in ROS production induced by DHFR knockdown. Intracellular BH2, as well as the relative concentrations of BH4 and BH2, together play a determining role in the redox regulation of eNOS-modulated endothelial responses [2]. 7,8-dihydro-L-biopterin was a reduced form of pterins. Pterins noncompetitively inhibited rat liver GTP cyclohydrolase I activity. 7,8-dihydro-L-biopterin exhibited approximately 12-times more potent than oxidized pterins. The Ki values for 7,8-dihydro-L-biopterin was 14.4 μM [1].
References:
[1] Shen R, Alam A, Zhang Y. Inhibition of GTP cyclohydrolase I by pterins[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 1988, 965(1): 9-15.
[2] Sugiyama T, Levy B D, Michel T. Tetrahydrobiopterin recycling, a key determinant of endothelial nitric-oxide synthase-dependent signaling pathways in cultured vascular endothelial cells[J]. Journal of Biological Chemistry, 2009, 284(19): 12691-12700.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 239.2 |
Cas No. | 6779-87-9 |
Formula | C9H13N5O3 |
Solubility | Soluble in DMSO |
Chemical Name | 2-amino-6-(1R,2S-dihydroxypropyl)-7,8-dihydro-4(1H)-pteridinone |
SDF | Download SDF |
Canonical SMILES | NC(N([H])C1=C2N=C([C@@H](O)[C@@H](O)C)CN1[H])=NC2=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |