Diethylmaleate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Diethylmaleate(DEM)是马来酸二乙酯。
DEM作为颜料、塑料、药物以及农产品的一种添加剂和中间体应用于许多有机合成中。 DEM也是生产粘合剂、油漆和共聚物的中间体。
体外:先前研究发现,DEM对GSH的消耗可以抑制转化细胞的生长。非细胞毒性剂量的DEM导致GSH消耗、细胞周期停滞、ROS产生、锚定非依赖性生长减少、细胞凋亡、基因表达改变以及所有三种MAPK家族成员的活化。此外,GSHe增加的胞内GSH水平可抵抗DEM的作用[1]。
体内:进行动物研究以评价DEM诱导的氧化应激对小鼠雄性生育力的作用。结果表明,腹腔注射DEM两周导致睾丸中还原型谷胱甘肽的减少和氧化型谷胱甘肽水平的增加。此外,研究发现精子的运动性和浓度显著降低,窝仔数明显减少。RT-PCR数据证明,在DEM处理后氧化还原敏感转录因子、cjun和cfos基因、γ-谷氨酰半胱氨酸合成酶和锰超氧化物歧化酶表达受到影响。此外,DEM治疗还引起SOD的酶活性增加[2]。
临床试验:目前尚无diethyl maleate的临床数据报道。
参考文献:
[1] Priya S,Nigam A,Bajpai P,Kumar S. Diethyl maleate inhibits MCA+TPA transformed cell growth via modulation of GSH, MAPK, and cancer pathways. Chem Biol Interact.2014 Aug 5;219:37-47.
[2] Kaur P,Kalia S,Bansal MP. Effect of diethyl maleate induced oxidative stress on male reproductive activity in mice: redox active enzymes and transcription factors expression. Mol Cell Biochem.2006 Oct;291(1-2):55-61.
Storage | Store at -20°C |
M.Wt | 172.18 |
Cas No. | 141-05-9 |
Formula | C8H12O4 |
Solubility | insoluble in H2O; ≥51 mg/mL in DMSO; ≥62.1 mg/mL in EtOH |
Chemical Name | diethyl maleate |
SDF | Download SDF |
Canonical SMILES | CCOC(/C=C\C(OCC)=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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