Bisindolylmaleimide V
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Bisindolylmaleimide V (BIM V, Ro 31-6045)是蛋白激酶C抑制活性的负对照，Kd值为100 µM [1]。Ro 31-6045也抑制S6K的活性，IC50值为8 µM [4]。
蛋白激酶C在多种介质跨越细胞膜的信号传导中发挥重要作用。PKC调节细胞增殖、分泌和基因表达。PKC的抑制可能治疗多种疾病，如类风湿关节炎、癌症和AIDS [2][3]。
Bisindolylmaleimide V是蛋白激酶C抑制剂Ro 31-8220的无活性类似物。Bisindolylmaleimide IV具有极弱的活性，只要在使用的最高浓度时抑制[3H]N-甲基东莨菪碱的结合[1]。Ro 31-6045也抑制丝裂原活化蛋白激酶p70s6k/p85s6k (S6K)的活性。在成纤维细胞中，Ro 31-6045抑制S6K的IC50值为8 µM [4]。
参考文献：
Lazareno S, Popham A, Birdsall NJ. Muscarinic interactions of bisindolylmaleimide analogues. Eur J Pharmacol. 1998 Nov 6;360(2-3):281-4.
Toullec D, Pianetti P, Coste H, et al. The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J Biol Chem. 1991 Aug 25;266(24):15771-81.
Davis PD, Hill CH, Lawton G, et al. Inhibitors of protein kinase C. 1. 2,3-Bisarylmaleimides. J Med Chem. 1992 Jan;35(1):177-84.
Marmy-Conus N, Hannan KM, Pearson RB. Ro 31-6045, the inactive analogue of the protein kinase C inhibitor Ro 31-8220, blocks in vivo activation of p70(s6k)/p85(s6k): implications for the analysis of S6K signalling. FEBS Lett. 2002 May 22;519(1-3):135-40.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 341.36 |
| Cas No. | 113963-68-1 |
| Formula | C21H15N3O2 |
| Solubility | Soluble in DMSO |
| Chemical Name | 3,4-di(1H-indol-3-yl)-1-methyl-1H-pyrrole-2,5-dione |
| SDF | Download SDF |
| Canonical SMILES | CN1C(C(C2=CNC3=CC=CC=C23)=C(C1=O)C4=CNC5=CC=CC=C45)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
