AS-252424
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AS-252424是一种新型有效的PI3Kγ小分子选择性抑制剂(IC50=30 nM).
PI3K(磷脂酰肌醇-4,5-二磷酸-3-激酶)是一类参与细胞功能如细胞生长\增殖\分化\运动\存活和胞内运输的酶,从而参与癌症.该物质在PI3K/Akt/mTOR通路中扮演关键角色.
在THP-1(人类单核细胞系)中,由于MCP1结合GPCR 受体CCR2,AS-252424抑制PKB/Akt磷酸化,最低IC50值为0.4 μM.在原代单核细胞和单核细胞野生型细胞系THP-1中,AS-252424可以浓度依赖的方式抑制MCP-1介导的趋化作用(IC50值分别为52 μM和53 μM).[1]在Capan 1和HPAF(胰腺癌细胞系)中,AS52424可特异性抑制细胞增殖.[2]
在巯基乙酸盐诱导腹膜炎小鼠模型中,口服10 mg/kg的AS-252424可适度降低中性粒细胞募集(35% ±14%).[1]
参考文献：
1. Pomel V, Klicic J, Covini D et al. Furan-2-ylmethylene thiazolidinediones as novel, potent, and selective inhibitors of phosphoinositide 3-kinase gamma. J Med Chem. 2006 Jun 29;49(13):3857-71.
2. Edling CE, Selvaggi F, Buus R et al. Key role of phosphoinositide 3-kinase class IB in pancreatic cancer. Clin Cancer Res. 2010 Oct 15;16(20):4928-37.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 305.28 |
| Cas No. | 900515-16-4 |
| Formula | C14H8FNO4S |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥30.5 mg/mL in DMSO |
| Chemical Name | (5Z)-5-[[5-(4-fluoro-2-hydroxyphenyl)furan-2-yl]methylidene]-1,3-thiazolidine-2,4-dione |
| SDF | Download SDF |
| Canonical SMILES | C1=CC(=C(C=C1F)O)C2=CC=C(O2)C=C3C(=O)NC(=O)S3 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
