ALX 5407 hydrochloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ALX 5407是高效选择性hGlyT1甘氨酸转运子抑制剂,其完全抑制GlyT1细胞中甘氨酸转运,IC50值为3 nM [1].
GlyT1是甘氨酸转运子家族中的一员.转运子终止甘氨酸和谷氨酸的活性.GlyT1家族中至少有3个剪接异构体,为1A\1B和1C.GlyT1在CNS中广泛分布,相比于strychnine敏感性甘氨酸受体,此分布与N-甲基-D-天冬氨酸(NMDA)受体的定位更加相关[1].
50 nM ALX 5407处理QT6-1C细胞,之后用HBS漂洗4次.漂洗之后加入90 μl HBS.随后测量ALX 5407从GlyT1C转运子中解离的速率,发现较长的半衰期.表明ALX 5407与GlyT1C转运子的结合基本上是不可逆的[1].
通过微透析发现,按10 mg/kg口服ALX 5407 60至90分钟后,大鼠前额叶皮层(PFC)中甘氨酸水平提高40%,而1 mg/kg的低剂量仅引起轻微和不显著的提高[1].
参考文献：
[1]. Atkinson BN, Bell SC, De Vivo M, et al. ALX 5407: a potent, selective inhibitor of the hGlyT1 glycine transporter. Mol Pharmacol, 2001, 60(6):1414-20.
| Physical Appearance | White solid |
| Storage | Store at -20°C |
| M.Wt | 429.92 |
| Cas No. | 200006-08-2 |
| Formula | C24H24FNO3·HCl |
| Solubility | <42.99mg/ml in DMSO; <21.5mg/ml in ethanol |
| Chemical Name | (S)-2-((3-([1,1'-biphenyl]-4-yloxy)-3-(4-fluorophenyl)propyl)(methyl)amino)acetic acid hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | FC1=CC=C(C=C1)[C@H](CCN(C)CC(O)=O)OC2=CC=C(C3=CC=CC=C3)C=C2.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
