Acetylcysteine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Tong Wang, Qi Sun, et al. "A1 Reactive Astrocytes Activated by 2-chloroethanol Modulate M1 Microglia Polarization through Upregulating IL-1β and TNF-α." Research Square. April 13th, 2021
- 2. Tong Wang, Qi Sun, et al. "A1 astrocytes activated by 2-Chloroethanol via the ROS-induced p38 MAPK/NF-κB and AP-1 pathways modulate microglia polarization." Research Square. April 12th, 2021.
- 3. PIUS REYDERG AGYEMANG. "Profifiling of FDA-Approved and Clinical Trial Drugs Revealed Shared Cytotoxicity And Collateral Sensitivity In Resistant (H69ar) And Non-Resistant (H69) Small Cell Lung Cancer Cells. (Drug Repurposing in Cancer Chemotherapy)." South Dakota State University 2021.
- 4. Jing Wu, Yan-Qiu You, et al. "DDX5-targeting fully human monoclonal autoantibody inhibits proliferation and promotes differentiation of acute promyelocytic leukemia cells by increasing ROS production." Cell Death & Disease volume 11, Article number: 552 (2020);20 July 2020. PMID: 32690860
- 5. Mahnaz Maddah, Mohammad A Mandegar, et al. "Quantifying drug-induced structural toxicity in hepatocytes and cardiomyocytes derived from hiPSCs using a deep learning method." J Pharmacol Toxicol Methods. 2020 Sep;105:106895. PMID: 32629158
- 6. Wang Z, Chen J, et al. "cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity." J Clin Invest. 2019 Aug 13;130:4850-4862. PMID: 31408442
- 7. Ye J, Xue M, et al. "Diosbulbin B-Induced Mitochondria-Dependent Apoptosis in L-02 Hepatocytes is Regulated by Reactive Oxygen Species-Mediated Autophagy." Front Pharmacol. 2019 Jun 19;10:676. PMID: 31275148
| Storage | Store at -20°C |
| M.Wt | 163.19 |
| Cas No. | 616-91-1 |
| Formula | C5H9NO3S |
| Synonyms | N-acetylcysteine; N-acetyl-L-cysteine; NAC; Acetadote |
| Solubility | ≥44.6 mg/mL in H2O; ≥53.3 mg/mL in EtOH; ≥8.16 mg/mL in DMSO |
| Chemical Name | (2R)-2-acetamido-3-sulfanylpropanoic acid |
| SDF | Download SDF |
| Canonical SMILES | CC(=O)NC(CS)C(=O)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]: | |
|
细胞系 |
PC12细胞 |
|
溶解方法 |
该化合物在DMSO中的溶解度> 10 mM。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
1–1000 μM; 3h |
|
应用 |
在PC12细胞中，Acetylcysteine (NAC)引起DOPAL水平的浓度依赖性降低。NAC（1-10μM）与selegiline（1μM）的共孵育减弱或阻止Cys-DA对selegiline的应答，而不干扰selegiline诱导的DOPAL产生减少或抑制酪氨酸羟化作用。 |
| 动物实验[2]: | |
|
动物模型 |
亨廷顿氏病（HD）R6/1转基因小鼠模型 |
|
剂量 |
500 mg/kg，腹腔注射, 4周 |
|
应用 |
在亨廷顿氏病（HD）R6/1转基因小鼠模型中，Acetylcysteine（NAC）-HD小鼠显示出强迫游泳试验（FST）的不动时间介于盐水-HD和盐水-WT小鼠之间。NAC表现出抗抑郁样作用。CPG（GLT-1的抑制剂）和NAC共同施用的HD小鼠显示比NAC-HD小鼠更高的不动时间，这表明NAC的抗抑郁样效应依赖于谷氨酸转运。 |
|
注意事项 |
请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同，这是由实验系统的误差引起的，属于正常现象。 |
|
References: [1]. Goldstein DS1, Jinsmaa Y2, Sullivan P2, et al. N-Acetylcysteine Prevents the Increase in Spontaneous Oxidation of Dopamine During Monoamine Oxidase Inhibition in PC12 Cells. Neurochem Res. 2017 Aug 24. [2] Wright DJ1,2, Gray LJ3,2, Finkelstein DI1, et al. N-acetylcysteine modulates glutamatergic dysfunction and depressive behavior in Huntington's disease. Hum Mol Genet. 2016 Jul 15;25(14):2923-2933. |
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质量控制和MSDS
- 批次:
化学结构
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