A 839977
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
A839977是新型的P2X7受体选择性拮抗剂,对人\大鼠和小鼠的IC50值分别为20 nM\42 nM和150 nM[1].
在稳定表达P2X7受体的132N1细胞中,A839977阻断BzATP诱导的胞内钙浓度变化,对人\大鼠和小鼠的IC50值分别为20 nM\42 nM和150 nM.在分化的人THP-1细胞中,A839977还抑制BzATP诱导的IL-1β释放以及YO-PRO摄取,其IC50分别为37 nM和7 nM.在BzATP浓度-钙内流效应曲线中,A839977使曲线平行右移,其pAα值为8.1.此外,A839977呈剂量依赖性地缓解CFA诱导的热痛觉过敏,其ED50值为100 μmol/kg(腹腔注射)[1].
参考文献：
[1] Honore P1, Donnelly-Roberts D, Namovic M, Zhong C, Wade C, Chandran P, Zhu C, Carroll W, Perez-Medrano A, Iwakura Y, Jarvis MF. The antihyperalgesic activity of a selective P2X7 receptor antagonist, A-839977, is lost in IL-1alphabeta knockout mice. Behav Brain Res. 2009 Dec 1;204(1):77-81.
| Physical Appearance | White solid |
| Storage | Store at -20°C |
| M.Wt | 413.26 |
| Cas No. | 870061-27-1 |
| Formula | C19H14Cl2N6O |
| Solubility | Soluble in DMSO |
| Chemical Name | (E)-N-(1-(2,3-dichlorophenyl)-1H-tetrazol-5(2H)-ylidene)-1-(2-(pyridin-2-yloxy)phenyl)methanamine |
| SDF | Download SDF |
| Canonical SMILES | ClC1=C(Cl)C(N2/C(N=NN2)=N\CC3=CC=CC=C3OC4=CC=CC=N4)=CC=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
