TMB substrate solution for ELISA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
TMB,即3, 3′,5 ,5′-Tetramethylbenzidine,是辣根过氧化物酶的常用底物。在辣根过氧化物酶或其他适当过氧化物酶的催化下,TMB会产生可溶性蓝色产物。在加入终止液后,可使用酶标仪进行检测。当使用H2SO4或者HCl作为终止液时,将产生黄色产物,通过使用酶标仪在 450 nm 处实现准确的强度测量。当使用NaF作为终止液时,在650nm处有较强光吸收,可在620-650nm测定吸光度。
通过辣根过氧化物酶(HRP)催化TMB显色,本产品可用于ELISA等实验的显色。本显色液也可以用于检测血液或血红蛋白等样品中的过氧化物酶含量。
产品货号 | 产品名称 | 规格 |
K1131 | TMB substrate solution for ELISA | 100 mL |
4°C避光保存,一年有效。 |