NP-40 Lysis Buffer
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NP-40裂解液(NP-40 Lysis Buffer)是一种比较温和的细胞组织裂解液,在非变性条件下裂解细胞或组织样品,从而制备蛋白样品。该产品的主要成分为50mM Tris(pH7.4),150mM NaCl,1% NP-40以及sodium pyrophosphate(焦磷酸钠),β-glycerophosphate(β-甘油磷酸酯),sodium orthovanadate(钒酸钠),sodium fluoride(氟化钠),EDTA,leupeptin(亮肽素)等多种抑制剂,可有效抑制蛋白的降解,维持原有蛋白间的相互作用。NP-40裂解液裂解得到的蛋白样品可以用于常规的PAGE、蛋白质免疫印迹(WB)、免疫沉淀(IP)、免疫共沉淀(co-IP)和酶联免疫吸附测定(ELISA)等。本产品可用于动物、植物的细胞或组织样品,也可以用于真菌或细菌样品。
产品货号 | 产品名称 | 规格 |
K1127 | NP-40 Lysis Buffer | 100 mL |
保存于-20°C,一年有效。 |