HotStart™ 2X SYBR Green qPCR Master Mix
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
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定量PCR（qPCR，又称Real-time PCR）是一种非常通用的的精确分析基因表达的技术。按方法不同可分为染料法和探针法两类，其中染料法更通用、更方便、成本更低。基于染料的qPCR法通过实时监测结合双链DNA的染料发射的荧光，可以在PCR的每个周期间接测量DNA扩增。当在某一个时间点上，检测到的荧光信号显著超过背景，就可以确定Ct值（Cq值）。所获得的Ct值可用于评估目标基因的相对丰度，也可根据适当的标准曲线计算绝对数量。
我们的产品HotStart™ 2X SYBR Green qPCR Master Mix在定量目标DNA或cDNA方面具有优越的特异性、强劲的扩增效率、理想的可重复性和稳定性。这是一个2X PreMix，使用了结合抗体的热启动Taq DNA聚合酶。理想的Taq聚合酶和合适的缓冲液保证了较好的特异性和较高的扩增速度。Mix中的SYBR Green I可嵌入双链DNA的双螺旋小沟区域，当它与每个周期扩增的双链DNA结合时，会发出绿色荧光，通过仪器监测荧光可以实时的间接定量扩增产物。
该试剂与两种不同浓度的ROX染料一起提供，用于对应的仪器，用来校正仪器中不同反应之间的荧光信号强度。在进行实验时，使用SYBR®或SYBR/FAM模式。
| Components | 1000 rxn with 10μL reaction 500 rxn with 20μL reaction 200 rxn with 50μL reaction |
5000 rxn with 10μL reaction 2500 rxn with 20μL reaction 1000 rxn with 50μL reaction |
10000 rxn with 10μL reaction 5000 rxn with 20μL reaction 2000 rxn with 50μL reaction |
| HotStart™ 2X SYBR Green qPCR Master Mix | |||
| 50X ROX Reference Dye (low concentration) | |||
| 50X ROX Reference Dye (high concentration) | |||
将所有组分存放在-20°C并避光保存一年以上。尽可能避免反复冻融。 | |||
通过热启动机制优化扩增特异性
卓越的灵敏度和准确性
在较宽的动态范围内，Ct值具有较高的可重复性
方便的2X PreMix，简化实验步骤