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SpCas9 mRNA (ARCA, 5mCTP, ψUTP)

Catalog No.
R1006
应用于基因编辑技术,和guideRNA协同对DNA进行定点切割。
组合的产品项目
规格价格库存 数量
100ug (1mg/mL)
¥ 1,570.00
Ship with 10-15 days
1mg (1mg/mL)
¥ 8,270.00
Ship with 10-15 days
5x1mg (1mg/mL)
¥ 29,770.00
Ship with 10-15 days

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Background

SpCas9 mRNA expresses an optimized version of Streptococcus pyogenes protein. It can be used with a purified guideRNA sequence for genomic DNA target recognition and cleavage in the CRISPR-Cas genome editing system. Type II CRISPR-Cas system from the bacterium S. pyogenes has been adapted for genome engineering in eukaryotic systems, which requires two main components: the endoculease Cas9 and non-coding guide RNA (gRNA). The Cas9 protein can be programmed with gRNA, targeting a site-specific DNA double-strand break (DSB). Our SpCas9 mRNA is provided at a concentration of 1mg/ml, it is ARCA capped, polyA-tailed and incorporated with modified nucleotides. The capping of ARCA (anti-reverse cap analog) ensures high translation efficacy, while the addition of 5mCTP/ψUTP and poly(A) tail reduces host cell immune response and enhance mRNA stability. Moreover, Cas9 mRNA expresses the Cas9 protein with two nuclear localization signal (NLS) and C terminal HA epitope tag that enhance the rate of DNA cleavage and facilitate downstream purification.

Description

mRNA Length 4521 nucleotides
Concentration1 mg/mL
Buffer 1 mM Sodium Citrate, pH 6.4 Storage -40°C or below
General tips请将其于冰上溶解,并小心防止RNase污染降解。 尽可能避免反复冻融。 不要涡旋震荡。 首次使用时,将其轻柔离心并分成几份,可供单独使用。 使用不含RNase的试剂和耗材,使用适当的无RNase技术。 直至与转染试剂混合,才可加入含有血清的培养基中。
Shipping Condition试用装:蓝冰运输。