EZ Cap™ mCherry mRNA (5mCTP, ψUTP)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
mCherry mRNA编码红色荧光蛋白mCherry。mCherry是红色荧光蛋白DsRed的衍生物,其从海葵(Discosoma)中分离得到。mCherry是一种单体荧光团,在587 nm处具有最大吸收峰,在610 nm处具有最大发射峰。mCherry具有良好的光稳定性,广泛用于生物学中的分子标记和细胞组分定位。
该mRNA使用共转录方式加帽,具有Cap 1结构。mCherry mRNA被聚腺苷酸化,并添加了5mCTP和ψUTP修饰,可以减少宿主细胞的免疫反应,提高mRNA的稳定性和翻译效率。
mRNA Length | 996 nucleotides | ||
Concentration | 1.0 mg/mL | ||
Buffer | 1 mM Sodium Citrate pH 6.4 | Storage | At or below -40°C |
Application | Reporter Genes | Cap | Cap 1 |