HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, ψUTP, T7, poly(A))
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, ψUTP, T7, poly(A)) 试剂盒用于在体外快速合成ARCA加帽，5mCTP和 ψUTP修饰，poly（A）加尾的mRNA。通过使用T7 RNA聚合酶，Cap0类似物（ARCA，货号B8175）以共转录方式被掺入mRNA 5'端，形成Cap0结构。ARCA的添加保护mRNA免于降解并确保高翻译效率。修饰核苷酸5mCTP（货号B7967）和ψUTP（Pseudo-UTP，货号B7972）的添加可以减少宿主细胞的免疫反应。经过短暂的DNase I处理以去除模板DNA后，加帽和修饰的mRNA可以利用Poly（A）聚合酶在3'端进行加尾。聚腺苷酸化（即Poly（A）尾的添加）使mRNA更加稳定并提高mRNA的翻译起始效率。
该试剂盒有诸多应用，例如体外翻译、反义RNA和RNAi实验、RNA疫苗，RNA结构和功能研究，核酶生物化学，RNase蛋白质实验和基于探针的杂交印迹。
每次标准反应为20 μL，该试剂盒可进行25次反应。对于每次的标准反应，1 μg 的对照模板可产生25-180 μg 的RNA。
（1）mRNA的合成（ARCA加帽，5mCTP和 ψUTP修饰） | |
| 组分 | 25 rxns |
| T7 RNA Polymerase Mix | 50 μL |
| 10X Reaction Buffer | 50 μL |
| ATP (20 mM) | 50 μL |
| UTP (20 mM) | 25 μL |
| CTP (20 mM) | 25 μL |
| GTP (20 mM) | 25 μL |
| ARCA (60 mM) | 35 μL |
| 5mCTP (10 mM) | 50 μL |
| Pseudo-UTP (10 mM) | 50 μL |
| Control Template (0.5 μg/μL) | 5 μL |
| RNase-free H2O | 0.5 mL |
所有的组分请在-20°C保存。 | |
（2）mRNA的加尾 | |
| 组分 | 25 rxns |
| E-PAP (2 units/μL) | 100 μL |
| 5X E-PAP Buffer | 600 μL |
| ATP Solution (100 mM) | 100 μL |
| 25 mM MnCl2 | 250 μL |
| Nuclease - free Water | 1 mL x 2 |
所有的组分请在-20°C保存。 | |
