WWL123
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 0.43 μM
WWL123 is a brain-penetrant inhibitor of ABHD6.
The serine hydrolase a/b-hydrolase domain 6 (ABHD6) has been reported to hydrolyze the most abundant endocannabinoid (eCB) in the brain, 2-arachidonoylglycerol (2-AG), and controls its availability at cannabinoid receptors.
In vitro: WWL123 was identified as a brain-penetrant inhibitor of ABHD6 demonstrating over 10-fold selectivity for ABHD6 compared to a panel of about 35 other serine hydrolases [1].
In vivo: In previous study, mice were pretreated with either vehicle or WWL123, and then they were treated with 50 mg/kg PTZ. It was found that pretreatment with WWL123 could block seizure-related mortality, reduce the severity of seizure behaviors, reduce the number of GTC seizures per mouse, and reduce the frequency of myoclonic (MC) seizures as well. In addition, to test whether behavioral seizures exhibited by R6/2 mice are controlled by chronic blockade of the eCB signaling system, the authors treated mice with a daily injection of vehicle, SR1, or WWL123. Results showed that WWL123 blocked the incidence of spontaneous behavioral seizures in R6/2 mice and there was no effect on duration of spontaneous seizures in response to chronic SR1 treatment vehicle [2].
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Bachovchin, D. A.,Ji, T.,Simon, G.M., et al. Superfamily-wide portrait of serine hydrolase inhibition achieved by library-versus-library screening. Proceedings of the National Academy of Sciences of the United States of America 107(49), 20941-20946 (2010).
[2] Naydenov, A. V.,Horne, E.A.,Cheah, C.S., et al. ABHD6 blockade exerts antiepileptic activity in PTZ-induced seizures and in spontaneous seizures in R6/2 mice. Neuron 83(2), 361-371 (2014).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 436.5 |
Cas No. | 1338575-41-9 |
Formula | C28H24N2O3 |
Solubility | ≤1mg/ml in DMSO |
Chemical Name | N-([1,1'-biphenyl]-3-ylmethyl)-N-methyl-carbamic acid, 4'-(aminocarbonyl)[1,1'-biphenyl]-3-yl ester |
SDF | Download SDF |
Canonical SMILES | O=C(N)C1=CC=C(C2=CC(OC(N(C)CC3=CC=CC(C4=CC=CC=C4)=C3)=O)=CC=C2)C=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
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